|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||2 µg/ml|
|Western Blot (WB)||See 2 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Dog , Rat Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: V(58) E D I N K R R E P I P S(70)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-742 detects Munc-18 from human, rat and mouse tissues.
PA1-742 has been successfully used in Western blot, immunohistochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects an ~67 kDa protein representing Munc-18 from rat and mouse whole brain extract.
PA1-742 immunizing peptide corresponds to amino acid residues 58-70 from human Munc-18. This sequence is completely conserved between human, mouse, rat, bovine, and canine Munc-18. PA1-742 immunizing peptide (Cat. # PEP-067) is available for use in neutralization and control experiments.
Munc-18 is a cytosolic brain protein of ~67 kDa which plays a key role in vesicle membrane fusion events with the plasma membrane. Functional interactions have been demonstrated to exist between Munc-18, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin, and syntaxin. Munc-18 is the mammalian homologue of the C. elegans gene unc-18. Mutations in Munc-18 can result in accumulations of acetylcholine and paralytic phenotype suggestive of a neurotransmitter release defect. Munc-18 thus appears to be required by the synaptic vesicle fusion complex. Two Munc-18 isoforms have recently been identified. Munc-18-1 has been shown to be primarily expressed in brain, while Munc-18-2 is expressed in most tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: hunc18; MGC146482; minisatellite 10g detected by probe MMS10; MUNC18-1; N-Sec1; neuronal SEC1; p67; Protein unc-18 homolog 1; Protein unc-18 homolog A; rbSec1; rop; Syntaxin-binding protein 1; UNC-18 homolog; unc-18-1; Unc-18A; unc18; Unc18-1
Gene Aliases: AI317162; AI326233; ANC18HA; MMS10-G; Ms10g; Munc-18a; MUNC18-1; N-sec1; NSEC1; NSEC1A; P67; Rb-sec1; RBSEC1; rbSec1A; rbSec1B; Sec1; STXBP1; Sxtbp1; UNC18; Unc18-1; UNC18A; Unc18h
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