Western blot analysis of Musashi-1 in human small intestine lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse small intestine, 4) rat small intestine and 5) mouse embryo brain lysate using a Musashi-1 polyclonal antibody (Product # PA5-23439) at 2, 2, 4 and 2 ug/ml, respectively. Goat anti-rabbit Ig HRP secondary antibody.
|Tested species reactivity||Dog, Horse, Human, Mouse, Non-human primate, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (233 TGLAPGYTYQFPEFRVER 250) of Musashi-1 protein.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||2-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Musashi-1 is a member of an evolutionarily conserved family of RNA-binding proteins that is prefentially expressed in the nervous system. It is a neural RNA-binding protein that is strongly expressed in fetal and adult neural stem cells (NSCs). Functionally, Musashi-1 plays important roles in cell fate decision, including the maintenance of the stem-cell state, differentiation and tumorigenesis. Musashi-1 is an important regulator of the Notch-1 pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.