Invitrogen

Musashi-1 Monoclonal Antibody (14H1), eBioscience™

Musashi-1 Antibody in Immunocytochemistry (ICC/IF)

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Musashi-1 Antibody (14-9896-82) in ICC/IF

Immunofluorescence analysis of Musashi-1 was performed using 70% confluent log phase Neural Stem cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with Musashi-1 Monoclonal Antibody (14H1), eBioscience™ (Product # 14-9896-82) at 1 µg/mL in 0.1% BSA, incu... View More

Musashi-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Musashi-1 Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))

Musashi-1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

14-9896-82

Applications

Tested Dilution

Publications

Western Blot (WB)

5 µg/mL

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1 µg/mL

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Amphibian, Human, Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

14H1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10598804

Product Specific Information

Description: This 14H1 monoclonal antibody recognizes human Musashi-1 (MSI-1), a 39 kDa RNA binding protein. Musashi-1 is expressed in CNS stem and progenitor cells with decreased expression in differentiating cells. Musashi-1 functions as a translational repressor, directly regulating its target proteins, Numb and p21 (CIP1). Musashi-1 has been shown to be expressed in stem cells in a variety of other tissues such as the gut, stomach, mammary gland, and hair follicles. In addition to normal tissue expression, Musashi-1 expression has been identified in cells from several tumor types, mainly pulmonary and adenocarcinoma, as well as large and small cell carcinomas. Recently, expression of Musashi-1 in ischemic lesions following stroke provides evidence for neurogenesis as well as the role of Musashi-1 in regulating apoptosis.

Musashi-1 is highly conserved and the 14H1 antibody also recognizes mouse and rat Musashi-1.

Applications Reported: This 14H1 antibody has been reported for use in immunoblotting (WB), immunohistology staining of paraffin embedded tissue sections, and immunocytochemistry.

Applications Tested: This 14H1 antibody has been tested by immunofluorescent staining of paraformaldehyde fixed and permeabilized cells. This can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Musashi-1 belongs to the Musashi family of RNA-binding proteins which play a role in cell fate determination, and maintenance of stem-cell state. Musashi family proteins are also involved in post transcriptional gene regulation. The gene encodes a protein with two conserved tandem RNA recognition motifs with highly conserved RNP (ribonucleoprotein) motifs. In mammals, Musashi-1 is expressed in fetal kidney, brain, liver and lung, and in adult brain and pancreas. In humans, the gene is located on chromosome 12.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The RNA-binding protein Msi2 regulates autophagy during myogenic differentiation.

Life science alliance

Wang R,Kato F,Watson RY,Beedle AM,Call JA,Tsunoda Y,Noda T,Tsuchiya T,Kashima M,Hattori A,Ito T

Published figure using Musashi-1 monoclonal antibody (Product # 14-9896-82) in Western Blot

Wed May 01 00:00:00 UTC 2024

Fibrinogen in the glioblastoma microenvironment contributes to the invasiveness of brain tumor-initiating cells.

Brain pathology (Zurich, Switzerland)

Dzikowski L,Mirzaei R,Sarkar S,Kumar M,Bose P,Bellail A,Hao C,Yong VW

Published figure using Musashi-1 monoclonal antibody (Product # 14-9896-82) in Immunocytochemistry

Wed Sep 01 00:00:00 UTC 2021

Fibrinogen in the glioblastoma microenvironment contributes to the invasiveness of brain tumor-initiating cells.

Brain pathology (Zurich, Switzerland)

Dzikowski L,Mirzaei R,Sarkar S,Kumar M,Bose P,Bellail A,Hao C,Yong VW

Published figure using Musashi-1 monoclonal antibody (Product # 14-9896-82) in Immunocytochemistry

Wed Sep 01 00:00:00 UTC 2021

Isolation and characterization of neural stem/progenitor cells in the subventricular zone of the naked mole-rat brain.

Inflammation and regeneration

Yamamura Y,Kawamura Y,Oiwa Y,Oka K,Onishi N,Saya H,Miura K

Published figure using Musashi-1 monoclonal antibody (Product # 14-9896-82) in Immunocytochemistry

Mon Nov 01 00:00:00 UTC 2021

PD-1 independent of PD-L1 ligation promotes glioblastoma growth through the NFκB pathway.

Science advances

Mirzaei R,Gordon A,Zemp FJ,Kumar M,Sarkar S,Luchman HA,Bellail AC,Hao C,Mahoney DJ,Dunn JF,Bose P,Yong VW

14-9896-82 was used in Immunocytochemistry to point to a critical role for PD-1 in BTICs and uncover a nonimmune resistance mechanism of patients with glioblastoma to PD-1- or PD-L1-blocking therapies.

Fri Nov 05 00:00:00 UTC 2021

Interaction of Discoidin Domain Receptor 1 with a 14-3-3-Beclin-1-Akt1 Complex Modulates Glioblastoma Therapy Sensitivity.

Cell reports

Vehlow A,Klapproth E,Jin S,Hannen R,Hauswald M,Bartsch JW,Nimsky C,Temme A,Leitinger B,Cordes N

14-9896-82 was used in Immunohistochemistry to identify a 14-3-3-Beclin-1-Akt1 protein complex assembling with DDR1 to be required for prosurvival Akt and mTOR signaling and regulation of autophagy-associated therapy sensitivity.

Tue Mar 26 00:00:00 UTC 2019

Spatio-temporal neural stem cell behavior leads to both perfect and imperfect structural brain regeneration in adult newts.

Biology open

Urata Y,Yamashita W,Inoue T,Agata K

14-9896 was used in Immunohistochemistry to indicate that newt brain regeneration is supported by modularity of boundary-domain neural stem cells with self-organising ability in neighbouring fields.

Mon Jun 25 00:00:00 UTC 2018

Inflammation-induced miRNA-155 inhibits self-renewal of neural stem cells via suppression of CCAAT/enhancer binding protein β (C/EBPβ) expression.

Scientific reports

Obora K,Onodera Y,Takehara T,Frampton J,Hasei J,Ozaki T,Teramura T,Fukuda K

Published figure using Musashi-1 monoclonal antibody (Product # 14-9896-82) in Western Blot

Mon Feb 27 00:00:00 UTC 2017

Inflammation-induced miRNA-155 inhibits self-renewal of neural stem cells via suppression of CCAAT/enhancer binding protein β (C/EBPβ) expression.

Scientific reports

Obora K,Onodera Y,Takehara T,Frampton J,Hasei J,Ozaki T,Teramura T,Fukuda K

Published figure using Musashi-1 monoclonal antibody (Product # 14-9896-82) in Western Blot

Mon Feb 27 00:00:00 UTC 2017

Image-based detection and targeting of therapy resistance in pancreatic adenocarcinoma.

Nature

Fox RG,Lytle NK,Jaquish DV,Park FD,Ito T,Bajaj J,Koechlein CS,Zimdahl B,Yano M,Kopp J,Kritzik M,Sicklick J,Sander M,Grandgenett PM,Hollingsworth MA,Shibata S,Pizzo D,Valasek M,Sasik R,Scadeng M,Okano H,Kim Y,MacLeod AR,Lowy AM,Reya T

Published figure using Musashi-1 monoclonal antibody (Product # 14-9896-82) in Immunofluorescence

Thu Jun 16 00:00:00 UTC 2016

Image-based detection and targeting of therapy resistance in pancreatic adenocarcinoma.

Nature

Published figure using Musashi-1 monoclonal antibody (Product # 14-9896-82) in Immunofluorescence

Thu Jun 16 00:00:00 UTC 2016

RNA-binding Protein Musashi Homologue 1 Regulates Kidney Fibrosis by Translational Inhibition of p21 and Numb mRNA.

The Journal of biological chemistry

Jadhav S,Ajay AK,Trivedi P,Seematti J,Pellegrini K,Craciun F,Vaidya VS

Published figure using Musashi-1 monoclonal antibody (Product # 14-9896-82) in Western Blot

Fri Jul 01 00:00:00 UTC 2016

RNA-binding Protein Musashi Homologue 1 Regulates Kidney Fibrosis by Translational Inhibition of p21 and Numb mRNA.

The Journal of biological chemistry

Jadhav S,Ajay AK,Trivedi P,Seematti J,Pellegrini K,Craciun F,Vaidya VS

Published figure using Musashi-1 monoclonal antibody (Product # 14-9896-82) in Western Blot

Fri Jul 01 00:00:00 UTC 2016

Role of RIP1 in physiological enterocyte turnover in mouse small intestine via nonapoptotic death.

Genes to cells : devoted to molecular & cellular mechanisms

Matsuoka Y,Tsujimoto Y

14-9896 was used in Immunohistochemistry to study the underlying mechanism of enterocyte shedding in the small intestine, as an example of programmed cell death.

Thu Jan 01 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: Musashi homolog 1(Drosophila); musashi RNA-binding protein 1; Musashi-1; musashi1; RNA-binding protein Musashi homolog 1

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Gene Aliases: m-Msi-1; MSI1; Msi1h; Musahi1

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UniProt ID: (Human) O43347, (Mouse) Q61474, (Rat) Q8K3P4

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Entrez Gene ID: (Human) 4440, (Mouse) 17690, (Rat) 259272

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Function(s)

nucleotide binding RNA binding poly(U) RNA binding poly(A) RNA binding nucleic acid binding single-stranded RNA binding RNA splicing factor

Process(es)

nervous system development response to hormone epithelial cell differentiation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Musashi-1 Monoclonal Antibody (14H1), eBioscience™

Musashi-1 Antibody (14-9896-82) in ICC/IF

Product Details

14-9896-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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