Invitrogen

Musashi-1 Monoclonal Antibody (14H1), eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

7 Published Figures

6 References

View all (35) MSI1 antibodies

Datasheet

Datasheet

Product Image Gallery

Musashi-1 Antibody — Figure 3 Over-expression of miR-155 leads to suppression of the self-renewal genes Msi1, Hes1 and Bmi1 and inhibition of self-renewal. ( A ) qPCR for Msi1, Hes1 and Bmi1 for NSCs transfected with the GFP-NTC (control) and the GFP-mmu-miR-155 (miR-155) plasmids. Asterisks represent significant differences (P < 0.05) compared with control. ( B ) Western blots for Msi1, Hes1 and Bmi1 for NSCs transfected with the control and miR-155 plasmids. ( C ) Western blot for caspase 3 for NSCs transfected with control and miR-155 plasmids. ( D ) Cumate induction of miR-155 and GFP expression in NSCs transfected with pPBQM-miR155-IRES-GFP. ( E ) qPCR for Msi1, Hes1 and Bmi1 for NSCs stably expressing pPBQM-miR155-IRES-GFP (QM-miR155). Asterisks represent significant differences (P < 0.05) among groups. ( F ) Rates of cell proliferation for NSCs stably expressing QM-miR155 with and without cumate treatment. Cell numbers were normalized by cell numbers for the control group at 3 days of culture. The asterisk represents a significant difference (P < 0.05).

Musashi-1 Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of Musashi-1 was performed using 70% confluent log phase Neural Stem cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with Musashi-1 Monoclonal Antibody (14H1), eBioscience™ (Product # 14-9896-82) at 1 ug/ml in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Product # A-11006), (1:2000 dilution) for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear and cytoplasmic localization. Panel e represents Neural Stem Cells differentiated to Astrocytes having no expression of Musashi-1. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Musashi-1 Antibody in Western Blot (WB) — Western blot was performed using Anti-Musashi-1 Monoclonal Antibody (14H1), eBioscience™ (Product # 14-9896-82) and a 40 kDa band corresponding to Musashi-1 was observed in Neural Stem Cells and IMR-32 and not in Neural Stem Cells differentiated to Astrocytes and MCF 10A. Whole cell extracts (30 µg lysate) of Neural Stem Cells (Lane 1), Neural Stem Cells differentiated to Astrocytes (Lane 2), IMR-32 (Lane 3) and MCF 10A (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (5 ug/ml) and detected by chemiluminescence with F(ab)2-Rabbit anti-Rat IgG (H+L) Secondary Antibody, HRP (Product # PA1-29927, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Musashi-1 Antibody in Relative expression — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. The expression was observed in Neural Stem Cells and IMR-32 and not in Neural Stem Cells differentiated to Astrocytes and MCF 10A using Musashi-1 Monoclonal Antibody (14H1), eBioscience™ (Product # 14-9896-80) in Western Blot. {RE}

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1 µg/mL

Immunofluorescence (IF)

1 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Western Blot (WB)

5 µg/mL

Published Applications

Immunohistochemistry (IHC)

See 2 publications below

Western Blot (WB)

See 2 publications below

Immunohistochemistry (Paraffin) (IHC (P))

See 1 publication below

Immunofluorescence (IF)

See 1 publication below

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Amphibian, Human, Mouse

Host / Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

14H1

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_10598804

Product Specific Information

Description: This 14H1 monoclonal antibody recognizes human Musashi-1 (MSI-1), a 39 kDa RNA binding protein. Musashi-1 is expressed in CNS stem and progenitor cells with decreased expression in differentiating cells. Musashi-1 functions as a translational repressor, directly regulating its target proteins, Numb and p21 (CIP1). Musashi-1 has been shown to be expressed in stem cells in a variety of other tissues such as the gut, stomach, mammary gland, and hair follicles. In addition to normal tissue expression, Musashi-1 expression has been identified in cells from several tumor types, mainly pulmonary and adenocarcinoma, as well as large and small cell carcinomas. Recently, expression of Musashi-1 in ischemic lesions following stroke provides evidence for neurogenesis as well as the role of Musashi-1 in regulating apoptosis.

Musashi-1 is highly conserved and the 14H1 antibody also recognizes mouse and rat Musashi-1.

Applications Reported: This 14H1 antibody has been reported for use in immunoblotting (WB), immunohistology staining of paraffin embedded tissue sections, and immunocytochemistry.

Applications Tested: This 14H1 antibody has been tested by immunofluorescent staining of paraformaldehyde fixed and permeabilized cells. This can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Musashi-1 belongs to the Musashi family of RNA-binding proteins which play a role in cell fate determination, and maintenance of stem-cell state. Musashi family proteins are also involved in post transcriptional gene regulation. The gene encodes a protein with two conserved tandem RNA recognition motifs with highly conserved RNP (ribonucleoprotein) motifs. In mammals, Musashi-1 is expressed in fetal kidney, brain, liver and lung, and in adult brain and pancreas. In humans, the gene is located on chromosome 12.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.