|Tested species reactivity||Tag|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide: EQKLISEEDL (Myc)|
|Storage buffer||PBS, pH 7.2|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Immunofluorescence (IF)||1:500 - 1:2000|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1000 - 1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-21316 detects N-terminal or C-terminal Myc tagged fusion proteins.
MA1-21316 has been successfully used in ELISA, immunofluorescence, immunoprecipitation and Western Blot procedures.
MA1-21316 immunogen is a synthetic peptide EQKLISEEDL (Myc).
MA1-21316 may be stored at 4°C short term. Add 0.05% sodium azide if desired. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.