Western blot analysis of Myc was performed by loading 50ug of NCCIT and Jurkat lysates and 10ug of Myc-MYCBP-containing cell lysate onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane. The membrane was blocked, and probed with an HRP-conjugated Myc Epitope Tag antibody (Product # MA1-21316-HRP) at a dilution of 1:1000 overnight at 4C. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075) at an exposure time of 2 minutes (NCCIT and Jurkat) and 3 seconds (Myc-MYCBP). Results show a band at ~57kDa (NCCIT and Jurkat) and 16kDa (Myc-MYCBP) as well as a 2 unknown bands at 35-40kDa.
|Tested species reactivity||Tag|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide: EQKLISEEDL (Myc)|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500 - 1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-21316-HRP has been successfully used in Western blotting applications on myc tagged samples.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.