HYT 27-02 detects Mycobacterium tuberculosis mycolytransferase. This antibody detects Mycobacterium tuberculosis Ag85C and Mycobacterium smegmatis homolog protein. This antibody does not cross react with Listeria monocytogenes or Escherichia coli.
HYT 27-02 has been successfully used in Western blotting and ELISA procedures. In ELISA, HYT 27-02 reacts strongly with M. tuberculosis cell cultrue filtrate or whole cells directly coated onto microtiter wells. In Western blots, HYT 27-02 reacts strongly with Ag85C and weaker with AG85A and Ag85B.
The HYT 27-02 immunogen is bacterial press extract from Mycobacterium tuberculosis H37Rv.
Epitope of HYT 27-02 is lacking in Purified Protein Derivitive or PPD (culture filtrate, heat treated and precipitated with trichloroacetic acid).
NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL
Tuberculosis is a chronic bacterial infection which remains one of the leading killers of young adults world wide. Mycobacterium tuberculosis enters the alveoli by airborne transmission. They are engulfed by alveolar macrophages but resist destruction. Although the exact mechanism is not known, it is suspected that that Mycrobacterium tuberculosis survives by preventing the fusion of the phagosome and lysosome. As the bacterium accumulates it forms a tubercle, spreads to the lymph nodes and becomes a systemic infection. Although treatments for tuberculosis do exist, new strains of Mycobacterium tuberculosis that appear to have multiple-drug resistance (MDR) pose a considerable threat to public health.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: M. tuberculosis; myobacter