|Tested species reactivity||Bacteria|
|Published species reactivity||Bacteria|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CFP10 (Rv3874) of Mycobacterium tuberculosis.|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.2 - 1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
This antibody recognizes CFP10 of Mycobacterium tuberculosis H37Rv and Mycobacterium bovis; it reacts also with recombinant antigen produced in Escherichia coli.
Tuberculosis is a chronic bacterial infection which remains one of the leading killers of young adults world wide. Mycobacterium tuberculosis enters the alveoli by airborne transmission. They are engulfed by alveolar macrophages but resist destruction. Although the exact mechanism is not known, it is suspected that that Mycrobacterium tuberculosis survives by preventing the fusion of the phagosome and lysosome. As the bacterium accumulates it forms a tubercle, spreads to the lymph nodes and becomes a systemic infection. Although treatments for tuberculosis do exist, new strains of Mycobacterium tuberculosis that appear to have multiple-drug resistance (MDR) pose a considerable threat to public health.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Correlation of phenotypic profiles using targeted proteomics identifies mycobacterial esx-1 substrates.
PA1-19445 was used in western blot to study 16 Esx-1-deficient Mycobacterium marinum strains to identify Esx-1 substrates
|Champion MM,Williams EA,Pinapati RS,Champion PA||Journal of proteome research (13:5151)||2014|