|Tested species reactivity||Bacteria|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||EsaT-6 (Rv3875) of Mycobacterium tuberculosis.|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.2-1.0 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunoprecipitation (IP)||See 1 publications below|
Tuberculosis is a chronic bacterial infection which remains one of the leading killers of young adults world wide. Mycobacterium tuberculosis enters the alveoli by airborne transmission. They are engulfed by alveolar macrophages but resist destruction. Although the exact mechanism is not known, it is suspected that that Mycrobacterium tuberculosis survives by preventing the fusion of the phagosome and lysosome. As the bacterium accumulates it forms a tubercle, spreads to the lymph nodes and becomes a systemic infection. Although treatments for tuberculosis do exist, new strains of Mycobacterium tuberculosis that appear to have multiple-drug resistance (MDR) pose a considerable threat to public health.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Mycobacterial shuttle vectors designed for high-level protein expression in infected macrophages.
PA1-19446 was used in immunoprecipitation to develop mycobacterial shuttle vectors for the high level expression of proteins by bacteria within infected macrophages
|Eitson JL,Medeiros JJ,Hoover AR,Srivastava S,Roybal KT,Aínsa JA,Hansen EJ,Gumbo T,van Oers NS||Applied and environmental microbiology (78:6829)||2012|