HYB 076-08-02 detects a 10-kDa band representing ESAT-6 (Rv3875) from short term culture of M. tuberculosis. Interspecies analyses with HYB 076-08 showed the presence of ESAT-6 in filtrates from M. tuberculosis complex species. Among filtrates from mycobacteria not belonging to the M. tuberculosis complex, reactivity was observed in Mycobacterium kansasii, Mycobacterium szulgai, and Mycobacterium marinum.
HYB 076-08-02 has been successfully used in Western blotting and ELISA procedures. HYB 076-08-02 reacts with PPD, culture filtrate, cytosol and cell wall preparations from M. tuberculosis.
HYB 076-08-02 binds to an epitope consisting of the residues E(3) Q Q W N F A G I E A A A(15) of ESAT-6.
NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL
Tuberculosis is a chronic bacterial infection which remains one of the leading killers of young adults world wide. Mycobacterium tuberculosis enters the alveoli by airborne transmission. They are engulfed by alveolar macrophages but resist destruction. Although the exact mechanism is not known, it is suspected that that Mycrobacterium tuberculosis survives by preventing the fusion of the phagosome and lysosome. As the bacterium accumulates it forms a tubercle, spreads to the lymph nodes and becomes a systemic infection. Although treatments for tuberculosis do exist, new strains of Mycobacterium tuberculosis that appear to have multiple-drug resistance (MDR) pose a considerable threat to public health.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: M. tuberculosis; myobacter