|Tested species reactivity||Bovine, Human, Mouse, Rat|
|Published species reactivity||Rat|
|Host / Isotype||Chicken / IgY|
|Immunogen||Full length purified myelin basic protein isolated from cow nerve|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In Western blot using rat spinal cord whole tissue homogenates, this antibody detects all isotypes of MPB, resulting in bands at ~21.5kDa, 18.5kDa, 17kDa and 14kDa.
Myelin Basic Protein (MBP) is one of the major proteins of the myelin sheath surrounding axons in the nervous system. Since it is of relatively low molecular weight and high abundance the protein sequence was determined from purified protein over 30 years ago. The protein is made by oligodendrocytes in the central nervous system so antibodies to MBP are good markers of this cell type. However, transcripts from the same gene are also expressed in certain hematopoetic lineage cells. In the central nervous system there are three different forms of the protein made by alternate transcription from a single gene, which have molecular weights of 21.5, 18.5 and 17.2 kDa. Since the two lower molecular weight forms are very close in molecular size, MBP antibodies typical show two bands on Western blots, one at about 22kDa and another at about 18 kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Regulation of later neurogenic stages of adult-derived neural stem/progenitor cells by L-type Ca2+ channels.
PA1-10008 was used in immunocytochemistry to determine which stage of neurogenesis is under the regulation of L-type Ca2+ channels
|Teh DB,Ishizuka T,Yawo H||Development, growth and differentiation (56:583)||2014|
Optogenetic patterning of whisker-barrel cortical system in transgenic rat expressing channelrhodopsin-2.
PA1-10008 was used in immunohistochemistry to develop a system that enables the precise spatiotemporal stimulation of the whisker-barrel cortical system using rats transgenically expressing channelrhodopsin-2
|Honjoh T,Ji ZG,Yokoyama Y,Sumiyoshi A,Shibuya Y,Matsuzaka Y,Kawashima R,Mushiake H,Ishizuka T,Yawo H||PloS one (9:null)||2014|
Light-evoked somatosensory perception of transgenic rats that express channelrhodopsin-2 in dorsal root ganglion cells.
PA1-10008 was used in immunohistochemistry to study light-evoked somatosensory perception in transgenic rats expressing channelrhodopsin-2
|Ji ZG,Ito S,Honjoh T,Ohta H,Ishizuka T,Fukazawa Y,Yawo H||PloS one (7:null)||2012|