|Tested species reactivity||Human, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues Y(68) G S L P Q K S Q R S Q D E N P V V(86) of guinea pig MBP, conjugated to KLH.|
|Storage buffer||whole serum|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||1:1,000 - 1:4,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA1-18324 detects MBP from human and rat samples.
PA1-18324 has been successfully used in immunohistochemistry staining of MBP in abnormal oligodendrocytic process and cell bodies in demyelinating areas. This antibody recognizes only areas of myelin degeneration when tested in injured spinal cord and lesioned sciatic nerve, and also stains discrete white matter in the brain of multiple system atrophy.
The PA1-18324 immunogen is a synthetic peptide corresponding to residues Y(68) G S L P Q K S Q R S Q D E N P V V(86) of guinea pig MBP, conjugated to KLH.
Reconstitute with 100 ul of distilled water.
Myelin is a membrane characteristic of the nervous tissue and functions as an insulator to increase the velocity of the stimuli being transmitted between a nerve cell body and its target. Myelin isolated from human and bovine nervous tissue is composed of approximately 80% lipid and 20% protein, and 30% of the protein fraction constitutes myelin basic protein. MBP is an 'intrinsically unstructured' protein with a high proportion (approximately 75%) of random coil, but postulated to have core elements of beta-sheet and alpha-helix. MBP is a major protein in CNS myelin and is expressed specifically in the nervous system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Fingerprint changes in CSF composition associated with different aetiologies in human neonatal hydrocephalus: glial proteins associated with cell damage and loss.
PA1-18324 was used in western blot to examine the protein profile of cerebrospinal fluid from human neonates with hydrocephalus
|Naureen I,Waheed KA,Rathore AW,Victor S,Mallucci C,Goodden JR,Chohan SN,Miyan JA||Fluids and barriers of the CNS (10:null)||2013|