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Western blot analysis of Myeloperoxidase using a Myeloperoxidase monoclonal antibody (Product # MA5-15480) against a truncated Trx-MPO recombinant protein (1), truncated MBP-MPO (aa1-193) recombinant protein (2) and truncated MPO (aa165-745) human IgG Fc transfected CHO-K1 cell lysate (3).
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant fragment of MPO (aa1-193) expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1/500 - 1/2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-15480 targets Myeloperoxidase in WB applications and shows reactivity with Human samples.
The MA5-15480 immunogen is purified recombinant fragment of MPO (aa1-193) expressed in E. Coli.
Myeloperoxidase is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of netrophils.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.