|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Native myeloperoxidase isolated from human leucocytes|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (PFA fixed) (IHC (PFA))||10-50 µg/ml|
|Western Blot (WB)||10 - 50 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Frozen) (IHC (F))||See 1 publications below|
Glycerol (1:1) may be added for additional stability.
Reconstitute with 500 ul of distilled water.
Centrifuge to remove any insoluble material.
Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of netrophils.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Chronic ethanol feeding promotes azoxymethane and dextran sulfate sodium-induced colonic tumorigenesis potentially by enhancing mucosal inflammation.
DOM00001G was used in immunohistochemistry - frozen section to study enhancing mucosal inflammation by chronic ethanol feeding that promotes azoxymethane and dextran sulfate sodium-induced colonic tumorigenesis
|Shukla PK,Chaudhry KK,Mir H,Gangwar R,Yadav N,Manda B,Meena AS,Rao R||BMC cancer (16:null)||2016|