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|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Myeloperoxidase isolated from human polymorphonuclear leucocytes.|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||1:500 - 1:1000|
|Western Blot (WB)||1:500 - 1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
PA1-18308 detects Myeloperoxidase from human samples.
PA1-18308 has been successfully used in immunohistochemistry (paraffin and frozen) and Western blot procedures.
The PA1-18308 immunogen is Myeloperoxidase isolated from human polymorphonuclear leucocytes.
Reconstitute with 250 ul of distilled water.
Myeloperoxidase is a hemoprotein that is abundantly expressed in neutrophils and secreted during their activation. Native Myeloperoxidase is represented as a covalently bound tetrameric complex of two glycosylated alpha chains (MW ~59 - 64 kDa) and two unglycosylated beta chains (MW ~14 kDa) with total MW ~150 kDa and theoretical pI 9.2. Traditionally Myeloperoxidase was considered as a main target of anti-neutrophil cytoplasm antibodies (ANCA), the serological markers for certain systemic vasculities e.g. periarteriitis nodosa, microscopic polyarteriitis and pulmonary eosinophilic granulomatosis (Churg-Strauss syndrome). Low to moderate anti-Myeloperoxidase autoantibody levels are also reported in rheumatoid arthritis. Recently it was shown that Myeloperoxidase participates in the initiation and progression of cardiovascular disease. It possesses potent proinflammatory properties and may contribute directly to tissue injury. Now Myeloperoxidase is under consideration as one of the most promising cardiac markers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Analysis of IL-17(+) cells in facet joints of patients with spondyloarthritis suggests that the innate immune pathway might be of greater relevance than the Th17-mediated adaptive immune response.
PA1-18308 was used in immunohistochemistry to compare the innate immune pathway with the Th17-mediated adaptive immune response
|Appel H,Maier R,Wu P,Scheer R,Hempfing A,Kayser R,Thiel A,Radbruch A,Loddenkemper C,Sieper J||Arthritis research & therapy (13:null)||2011|