Anti-Myeloperoxidase (Human Leukocytes) detects multiple MPO subunits and chain combinations by Western blot. Polyclonal rabbit-anti-Myeloperoxidase (Product # PA1-26402) was used at a 1:5000 dilution to detect 1.0 ug of human myeloperoxidase. This antibody detects a multiple bands corresponding to 53 kDa and 15 kDa polypeptides and chain combinations forming 68 kDa and 106 kDa proteins. The staining of the 68 kDa band is so intense that is over saturates the signal detection. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody for 2 h at room temperature followed by washes and reaction with a 1:5000 dilution of infrared 800 conjugated Goat-anti-Rabbit IgG [H and L] for 30 min at room temperature.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Myeloperoxidase from human white cells.|
|Storage buffer||whole serum diluted in 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-26402 detects Myeloperoxidase from human samples.
PA1-26402 has been successfully used in ELISA and Western blot procedures.
The PA1-26402 immunogen is Myeloperoxidase from human white cells.
Store at 4°C short term. For extended storage, aliquot and store at -20°C or below. Avoid freeze/thaw cycles.
Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of netrophils.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.