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Immunohistochemistry was performed on formalin -fixed paraffin-embedded rat infarct heart tissue sections. To expose target proteins, heat-induced epitope retrieval (HIER) was performed in sodium citrate buffer (pH 6.0) for 20 minutes at 100°C. Tissues were blocked in 10% normal goat serum for 20 minutes at room temperature and probed with a myeloperoxidase polyclonal antibody (Product # PA5-16672) at a dilution of 1:200 in 1% normal goat serum for 1hour at room temperature. Tissues were washed extensively with 1X PBS. Detection was performed using a biotinylated goat anti-rabbit IgG secondary antibody followed by an avidin-biotin complex reagent and DAB colorimetric substrate. Tissues were visualized by light microscopy. Data courtesy of the Innovators Program.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Ferret, Rat, Mouse, Human, Rhesus monkey|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Purified human granulocytic MPO|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16672 targets Myeloperoxidase in IHC (P) applications and shows reactivity with Human, mouse, and Rat samples.
The PA5-16672 immunogen is purified human granulocytic MPO.
PA5-16672 detects MPO-DNA complexes in purified PMA-induced human neutrophil extracellular traps in ELISA application.
Myeloperoxidase is an important enzyme used by granulocytes during phagocytic lysis of foreign particles engulfed. In normal tissues and in a variety of myeloproliferative disorders myeloid cells of both neutrophilic and eosinophilic types, at all stages of maturation, exhibit strong cytoplasmic reactivity for MPO. Erythroid precursors, megakaryocytes, lymphoid cells, mast cells, and plasma cells are nonreactive. MPO is not observed in the neoplastic cells of a wide variety of epithelial tumors and sarcomas. MPO is useful in differentiating between myeloid and lymphoid leukemias.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Genetic deletion of the prostaglandin E2 E prostanoid receptor subtype 3 improves anatomical and functional outcomes after intracerebral hemorrhage.
PA5-16672 was used in immunohistochemistry - paraffin section to study the contribution of PGE2 -EP3 signaling in modulating anatomical outcomes and functional recovery following intracerebral hemorrhage
|Leclerc JL,Lampert AS,Diller MA,Doré S||The European journal of neuroscience (41:1381)||2015|
Statins improve the resolution of established murine venous thrombosis: reductions in thrombus burden and vein wall scarring.
PA5-16672 was used in western blot to test the effects of statins in decreasing thrombus burden and decreasing vein wall injury in established murine stasis and non-stasis chemical-induced venous thrombosis
|Kessinger CW,Kim JW,Henke PK,Thompson B,McCarthy JR,Hara T,Sillesen M,Margey RJ,Libby P,Weissleder R,Lin CP,Jaffer FA||PloS one (10:null)||2015|
Improving effect of Sivelestat on lipopolysaccharide-induced lung injury in rats.
PA5-16672 was used in immunohistochemistry to study the protective effect of the neutrophil elastase inhibitor Sivelestat against LPS-induced rat lung injury
|Yuan Q,Jiang YW,Fang QH||APMIS : acta pathologica, microbiologica, et immunologica Scandinavica (122:810)||2014|
Glycyrrhizin inhibits the inflammatory response in mouse mammary epithelial cells and a mouse mastitis model.
PA5-16672 was used in immunohistochemistry to study the molecular mechanisms underlying the anti-inflammatory actions of glycyrrhizin in murine mammary cells and in vivo in a murine mastitis model
|Fu Y,Zhou E,Wei Z,Liang D,Wang W,Wang T,Guo M,Zhang N,Yang Z||The FEBS journal (281:2543)||2014|
A concomitant loss of dormant origins and FANCC exacerbates genome instability by impairing DNA replication fork progression.
PA5-16672 was used in immunohistochemistry to study the role of impaired progression of the DNA replication fork in the mechanism by which dormant origin loss and FANCC deletion lead to increased genomic instability
|Luebben SW,Kawabata T,Johnson CS,O'Sullivan MG,Shima N||Nucleic acids research (42:5605)||2014|
Overexpression of GATA-3 in T cells accelerates dextran sulfate sodium-induced colitis.
PA5-16672 was used in immunohistochemistry to study the increased severity of dextran sulfate-induced colitis in mice whose T-cells transgenically overexpress GATA-3
|Okamura M,Yoh K,Ojima M,Morito N,Takahashi S||Experimental animals / Japanese Association for Laboratory Animal Science (63:133)||2014|
Microbial symbionts accelerate wound healing via the neuropeptide hormone oxytocin.
PA5-16672 was used in immunohistochemistry to study the role of oxytocin upregulatin in the mechanism by which symbiotic gut bacteria accelerate mammalian wound healing
|Poutahidis T,Kearney SM,Levkovich T,Qi P,Varian BJ,Lakritz JR,Ibrahim YM,Chatzigiagkos A,Alm EJ,Erdman SE||PloS one (8:null)||2013|
CXC chemokine KC fails to induce neutrophil infiltration and neoangiogenesis in a mouse model of myocardial infarction.
PA5-16672 was used in immunohistochemistry to study whether the CXC chemokine KC has any effect on inflammation and wound repair in a murine model of myocardial infarction
|Oral H,Kanzler I,Tuchscheerer N,Curaj A,Simsekyilmaz S,Sönmez TT,Radu E,Postea O,Weber C,Schuh A,Liehn EA||Journal of molecular and cellular cardiology (60:1)||2013|
Assessment of ultraviolet B-blocking effects of weekly disposable contact lenses on corneal surface in a mouse model.
PA5-16672 was used in immunohistochemistry to study the ability of different weekly disposable contact lenses to protect against UVB irradiation using a murine corneal surface model
|Lin DP,Chang HH,Yang LC,Huang TP,Liu HJ,Chang LS,Lin CH,Chen BY||Molecular vision (19:1158)||2013|
Layer V cortical neurons require microglial support for survival during postnatal development.
PA5-16672 was used in immunohistochemistry to study the role of microglial cells in the post-natal development of layer V cortical neurons
|Ueno M,Fujita Y,Tanaka T,Nakamura Y,Kikuta J,Ishii M,Yamashita T||Nature neuroscience (16:543)||2013|
Prevention of hyperoxia-mediated pulmonary inflammation in neonatal rats by caffeine.
PA5-16672 was used in immunohistochemistry to study the ability of caffeine to protect against pulmonary inflammation caused by hyperoxia in neonatal rats
|Weichelt U,Cay R,Schmitz T,Strauss E,Sifringer M,Bührer C,Endesfelder S||The European respiratory journal (41:966)||2013|
Ambrisentan reduces pulmonary arterial hypertension but does not stimulate alveolar and vascular development in neonatal rats with hyperoxic lung injury.
PA5-16672 was used in immunohistochemistry to study the beneficial effects of an endothelin receptor type A antagonist on pulmonary arterial hypertension in a murine model of neonatal chronic lung disease
|Wagenaar GT,Laghmani el H,de Visser YP,Sengers RM,Steendijk P,Baelde HJ,Walther FJ||American journal of physiology. Lung cellular and molecular physiology (304:L264)||2013|
Xist RNA is a potent suppressor of hematologic cancer in mice.
PA5-16672 was used in immunohistochemistry to study the role of Xist RNA in suppressing the development of hematological cancers using mice deleted for Xist in the blood compartment
|Yildirim E,Kirby JE,Brown DE,Mercier FE,Sadreyev RI,Scadden DT,Lee JT||Cell (152:727)||2013|
Fish oil attenuates omega-6 polyunsaturated fatty acid-induced dysbiosis and infectious colitis but impairs LPS dephosphorylation activity causing sepsis.
PA5-16672 was used in immunohistochemistry to study the mechanisms underlying the ability of fish oil to protect against omega-6 PUFA-induced colitis but to increase the susceptibility to sepsis
|Ghosh S,DeCoffe D,Brown K,Rajendiran E,Estaki M,Dai C,Yip A,Gibson DL||PloS one (8:null)||2013|
Cannabinoid receptor 1 suppresses transient receptor potential vanilloid 1-induced inflammatory responses to corneal injury.
PA5-16672 was used in immunohistochemistry to study the mechanism by which injury-induced activativation of CB1 receptors leads to downregulation of corneal TRPV1-induced inflammation
|Yang Y,Yang H,Wang Z,Varadaraj K,Kumari SS,Mergler S,Okada Y,Saika S,Kingsley PJ,Marnett LJ,Reinach PS||Cellular signalling (25:501)||2013|
The effectiveness of heliox in acute respiratory distress syndrome.
PA5-16672 was used in immunohistochemistry to study acute respiratory distress syndrome and the therapeutic efficacy of heliox treatment
|Yilmaz S,Daglioglu K,Yildizdas D,Bayram I,Gumurdulu D,Polat S||Annals of thoracic medicine (8:46)||2013|
Prevention of hypoglycemia-induced neuronal death by minocycline.
PA5-16672 was used in immunohistochemistry to study the ability of minocycline to protect against the induction of neuronal cell death by hypoglycemia
|Won SJ,Kim JH,Yoo BH,Sohn M,Kauppinen TM,Park MS,Kwon HJ,Liu J,Suh SW||Journal of neuroinflammation (9:null)||2012|
Comparative transcriptomic analyses of atopic dermatitis and psoriasis reveal shared neutrophilic inflammation.
PA5-16672 was used in immunohistochemistry to perform a comparative study of atopic dermatitis and psoriasis at the level of the transcriptome and to examine the involvement of neutrophilic inflammation
|Choy DF,Hsu DK,Seshasayee D,Fung MA,Modrusan Z,Martin F,Liu FT,Arron JR||The Journal of allergy and clinical immunology (130:1335)||2012|
MyD88 signaling promotes both mucosal homeostatic and fibrotic responses during Salmonella-induced colitis.
PA5-16672 was used in immunohistochemistry to study the role of MyD88 signaling in the response to Salmonella-induced colitis
|Månsson LE,Montero M,Zarepour M,Bergstrom KS,Ma C,Huang T,Man C,Grassl GA,Vallance BA||American journal of physiology. Gastrointestinal and liver physiology (303:G311)||2012|
MMP-8 deficiency increases TLR/RAGE ligands S100A8 and S100A9 and exacerbates lung inflammation during endotoxemia.
PA5-16672 was used in immunohistochemistry to study the effects of MMP8 deficiency on TLR/RAGE ligands and lung inflammation during endotoxemia in mice
|González-López A,Aguirre A,López-Alonso I,Amado L,Astudillo A,Fernández-García MS,Suárez MF,Batalla-Solís E,Colado E,Albaiceta GM||PloS one (7:null)||2012|
Myeloperoxidase inhibition ameliorates multiple system atrophy-like degeneration in a transgenic mouse model.
PA5-16672 was used in immunohistochemistry to study the effect of myeloperoxidase inhibition in a murine transgenic model of multiple system atrophy-like degeneration
|Stefanova N,Georgievska B,Eriksson H,Poewe W,Wenning GK||Neurotoxicity research (21:393)||2012|
Signal transducer and activator of transcription-3/suppressor of cytokine signaling-3 (STAT3/SOCS3) axis in myeloid cells regulates neuroinflammation.
PA5-16672 was used in immunohistochemistry to study the modulation of neuroinflammation by myeloid cell SOCS3 and STAT3
|Qin H,Yeh WI,De Sarno P,Holdbrooks AT,Liu Y,Muldowney MT,Reynolds SL,Yanagisawa LL,Fox TH,Park K,Harrington LE,Raman C,Benveniste EN||Proceedings of the National Academy of Sciences of the United States of America (109:5004)||2012|
Vasculitis: molecular imaging by targeting the inflammatory enzyme myeloperoxidase.
PA5-16672 was used in immunohistochemistry to study the utility of molecular imaging of myeloperoxidase to non-invasively detect vasculitis-associated inflammation
|Su HS,Nahrendorf M,Panizzi P,Breckwoldt MO,Rodriguez E,Iwamoto Y,Aikawa E,Weissleder R,Chen JW||Radiology (262:181)||2012|
Protective role of Akt2 in Salmonella enterica serovar typhimurium-induced gastroenterocolitis.
PA5-16672 was used in immunohistochemistry to investigate the protective effect of Akt2 against gastroenterocolitis induced by Salmonella infection
|Kum WW,Lo BC,Yu HB,Finlay BB||Infection and immunity (79:2554)||2011|
Resistance to bleomycin-induced lung fibrosis in MMP-8 deficient mice is mediated by interleukin-10.
PA5-16672 was used in immunohistochemistry to study the role of IL-10 in the resistance to bleomycin-induced lung fibrosis in MMP-8-deficient mice
|García-Prieto E,González-López A,Cabrera S,Astudillo A,Gutiérrez-Fernández A,Fanjul-Fernandez M,Batalla-Solís E,Puente XS,Fueyo A,López-Otín C,Albaiceta GM||PloS one (5:null)||2010|
Impaired innate immune response and enhanced pathology during Citrobacter rodentium infection in mice lacking functional P-selectin.
PA5-16672 was used in immunohistochemistry to investigate the influence of P-selectin deletion on innate immunity and Citrobacter infection
|Kum WW,Lo BC,Deng W,Ziltener HJ,Finlay BB||Cellular microbiology (12:1250)||2010|
Carbohydrate determinants in ferret conjunctiva are affected by infection with influenza H1N1 virus.
PA5-16672 was used in immunoprecipitation to study the effects of influenza H1N1 infection on carbohydrate determinants of the ferret conjunctiva
|Kirkeby S,Martel CJ,Aasted B,Vorum H||Current eye research (38:1027)||2013|
Myeloperoxidase induces the priming of platelets.
PA5-16672 was used in immunocytochemistry to investigate the role of myeloperoxidase in the function of platelets
|Kolarova H,Klinke A,Kremserova S,Adam M,Pekarova M,Baldus S,Eiserich JP,Kubala L||Free radical biology & medicine (61:357)||2013|
Interleukin-17A is present in neutrophils in endometrioma and stimulates the secretion of growth-regulated oncogene-α (Gro-α) from endometrioma stromal cells.
PA5-16672 was used in immunocytochemistry to study the ability of endometrial neutrophil IL-17A to stimulate endometrial stromal cells to secrete Gro-alpha
|Takamura M,Osuga Y,Izumi G,Yoshino O,Koga K,Saito A,Hirata T,Hirota Y,Harada M,Hasegawa A,Taketani Y||Fertility and sterility (98:1218)||2012|
Immunohistochemical analysis on cyclooxygenase-2 for wound age determination.
PA5-16672 was used in immunocytochemistry to study the value of the immunohistochemical expression of COX-2 for determining the age of wounds
|Ishida Y,Kimura A,Nosaka M,Kuninaka Y,Takayasu T,Eisenmenger W,Kondo T||International journal of legal medicine (126:435)||2012|