|Tested species reactivity||Human|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to C-terminus of human myeloperoxidase protein.|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil tissue.
Myeloperoxidase (MPO) is an important enzyme used by granulocytes during phagocytic lysis of engulfed foreign particles. In normal tissues and in a variety of myeloproliferative disorders myeloid cells of both neutrophilic and eosinophilic types, at all stages of maturation, exhibit strong cytoplasmic reactivity for MPO. Erythroid precursors, megakaryocytes, lymphoid cells, mast cells, and plasma cells are nonreactive. MPO is not observed in epithelial tumors and sarcomas. MPO is positive in myeloid leukemias, but negative in lymphoid leukemias.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Potential roles of interleukin-17A in the development of skin fibrosis in mice.
MA5-16383 was used in immunohistochemistry to study murine skin fibrosis and the potential involvement of IL-17A
|Okamoto Y,Hasegawa M,Matsushita T,Hamaguchi Y,Huu DL,Iwakura Y,Fujimoto M,Takehara K||Arthritis and rheumatism (64:3726)||2012|