Invitrogen

Myeloperoxidase Recombinant Rabbit Monoclonal Antibody (JM10-58)

Product Image Gallery

Myeloperoxidase Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of Myeloperoxidase in MCF-7 cells using a Myeloperoxidase Monoclonal antibody (Product # MA5-32582) as seen in red. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Myeloperoxidase Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Myeloperoxidase of paraffin-embedded Human tonsil tissue using a Myeloperoxidase Monoclonal antibody (Product #MA5-32582). Counter stained with hematoxylin.

Myeloperoxidase Antibody in Western Blot (WB) — Western blot analysis of Myeloperoxidase in HL-60 cell lysate using a Myeloperoxidase Monoclonal antibody (Product # MA5-32582) at a dilution of 1:1,000.

Product Details

MA5-32582

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	-
Immunohistochemistry (IHC)	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:200	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Recombinant Monoclonal
Type	Antibody
Clone	JM10-58
Immunogen	Synthetic peptide within Human Myeloperoxidase aa 178-219 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2809859

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Myeloperoxidase (MPO) is a hemoprotein that is abundantly expressed in neutrophils and secreted during their activation. Native Myeloperoxidase is represented as a covalently bound tetrameric complex of two glycosylated alpha chains with a molecular weight range of 59 - 64 kDa, and two unglycosylated beta chains (MW ~14 kDa) with total MW ~150 kDa and a theoretical pI of 9.2. Traditionally, myeloperoxidase was considered as a main target of anti-neutrophil cytoplasm antibodies (ANCA), the serological markers for certain systemic vasculities such as periarteriitis nodosa, microscopic polyarteriitis and pulmonary eosinophilic granulomatosis (Churg-Strauss syndrome). Low to moderate anti-myeloperoxidase autoantibody levels are also reported in rheumatoid arthritis. Recently, it was shown that myeloperoxidase participates in the initiation and progression of cardiovascular disease. Myeloperoxidase possesses potent proinflammatory properties and may contribute directly to tissue injury. Now Myeloperoxidase is under consideration as one of the most promising cardiac markers. Myeloperoxidase is part of the host defense system of polymorphonuclear leukocytes and myeloperoxidase is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN (polymorphonuclear leukocytes), MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.