|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant protein containing rat myogenin aa 30-224|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Gel Shift (GS)||1mg/mL|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Immunoprecipitation (IP)||3 ug/mg protein lysate|
|Western Blot (WB)||1-2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-11658 targets Myogenin in GS, IF, IHC (P), IP, and WB applications and shows reactivity with Human, mouse, and Rat samples.
The MA5-11658 immunogen is recombinant protein containing rat myogenin aa 30-224.
Myogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Transfection of myogenin into multipotential mesodermal cells have been shown to convert the mesodermal cells to myoblasts. Expression of myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.