Human skeletal muscle myoblasts (hSMM) fixed in 3.7% formaldehyde and permeabilized with 0.2% Triton X-100 were stained with either Myogenin Monoclonal Antibody(Product # MA5-11486) at 1:10 dilution and an anti-mouse Texas Red secondary antibody (left) or secondary antibody alone (right). Nuclei were stained with DAPI (blue). Data courtesy of Innovators Program.
|Tested species reactivity||Cat, Human, Mouse, Rat|
|Published species reactivity||Rat, Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant protein containing rat myogenin aa 30-224|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Gel Shift (GS)||1mg/mL|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:400-1:800|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
MA5-11486 targets Myogenin in GS, IF, IHC (P), IP, and WB applications and shows reactivity with Feline, Human, mouse, and Rat samples.
The MA5-11486 immunogen is recombinant protein containing rat myogenin aa 30-224.
Myogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Transfection of myogenin into multipotential mesodermal cells have been shown to convert the mesodermal cells to myoblasts. Expression of myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas.
Molecular characterization of an EWSR1-POU5F1 fusion associated with a t(6;22) in an undifferentiated soft tissue sarcoma.
MA5-11486 was used in immunohistochemistry to characterize a clinical case of undifferentiated soft tissue sarcoma with chromosomal rearrangement and resultant EWSR1-POU5F1 fusion
|Deng FM,Galvan K,de la Roza G,Zhang S,Souid AK,Stein CK||Cancer genetics (204:423)||2011|
Mesenchymal stem cells improve the healing of ischemic colonic anastomoses (experimental study).
MA5-11486 was used in immunohistochemistry to study the role of allogenic mesenchymal stem cell transplantation in improved healing of ischemic colon anastomosis
|Adas G,Arikan S,Karatepe O,Kemik O,Ayhan S,Karaoz E,Kamali G,Eryasar B,Ustek D||Langenbeck's archives of surgery / Deutsche Gesellschaft fu¿r Chirurgie (396:115)||2011|
Endometriosis-associated skeletal muscle regeneration: a hitherto undescribed entity and a potential diagnostic pitfall.
MA5-11486 was used in immunohistochemistry to investigate the association between endometriosis and skeletal muscle regeneration
|Colella R,Mameli MG,Bellezza G,Sordo RD,Cavaliere A,Sidoni A||The American journal of surgical pathology (34:10)||2010|
Pulmonary meningothelial-like nodules: new insights into a common but poorly understood entity.
MA5-11486 was used in immunohistochemistry to study pulmonary meningothelial-like nodules
|Mukhopadhyay S,El-Zammar OA,Katzenstein AL||The American journal of surgical pathology (33:487)||2009|
A comprehensive characterization study of human bone marrow mscs with an emphasis on molecular and ultrastructural properties.
MA5-11486 was used in immunocytochemistry to study the molecular and ultrastructural properties of human bone marrow-derived mesenchymal stem cells
|Karaöz E,Okçu A,Gacar G,Sağlam O,Yürüker S,Kenar H||Journal of cellular physiology (226:1367)||2011|
Isolation and in vitro characterisation of dental pulp stem cells from natal teeth.
MA5-11486 was used in immunocytochemistry to characterize the biological properties and differentiation potential of human natal dental pulp stem cells
|Karaöz E,Doğan BN,Aksoy A,Gacar G,Akyüz S,Ayhan S,Genç ZS,Yürüker S,Duruksu G,Demircan PC,Sariboyaci AE||Histochemistry and cell biology (133:95)||2010|
Characterization of mesenchymal stem cells from rat bone marrow: ultrastructural properties, differentiation potential and immunophenotypic markers.
MA5-11486 was used in immunocytochemistry to study the morphology and immunology of rat bone marrow-derived mesenchymal stem cells
|Karaoz E,Aksoy A,Ayhan S,Sariboyaci AE,Kaymaz F,Kasap M||Histochemistry and cell biology (132:533)||2009|
class C basic helix-loop-helix protein 3; myf-4; MYOD1-related protein; myogenic factor 4; Myogenic factor-4; myogenin
BHLHC3; myf-4; MYF4; myo; MYOG