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|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant protein encoding rat myogenin aa 30-224.|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:40|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-20978 detects Myogenin in Human, Mouse and Rat samples.
MA1-20978 has been successfully used in Immunohistochemistry (Paraffin), Immunoprecipitation and Western Blot procedures.
MA1-20978 immunogen corresponds to Recombinant protein encoding rat myogenin aa 30-224..
Myogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Transfection of myogenin into multipotential mesodermal cells have been shown to convert the mesodermal cells to myoblasts. Expression of myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
bHLHc3; class C basic helix-loop-helix protein 3; MYF4; MYOD1-related protein; myogenic factor 4; MYOGENIN
BHLHC3; myf-4; MYF4; myo; MYOG