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Western blot analysis of Myosin 6 in (lane 1) A431 (lane 2) MCF-7 (lane 3) MDA-MB-231 lysates. Samples were separated on SDS-PAGE and probed with a Myosin 6 monoclonal antibody (Product # MA1-24948) at a dilution of 0.5 µg/ml. The antibody was developed using a Goat Anti-mouse IgG-Peroxidase and a chemiluminescent substrate.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide corresponding to residues 291-302 of human MYO6.|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-24948 detects MYO6 from human, mouse, and rat samples.
MA1-24948 has been successfully used in Western blot and ELISA procedures. Detects a band ~150 kDa, A suggested positive control for this product is A431 whole cell lysate.
The MA1-24948 immunogen is a synthetic peptide corresponding to residues 291-302 of human MYO6.
This gene is one of three myosin V heavy-chain genes, belonging to the myosin gene superfamily. Myosin V is a class of actin-based motor proteins involved in cytoplasmic vesicle transport and anchorage, spindle-pole alignment and mRNA translocation. The protein encoded by this gene is abundant in melanocytes and nerve cells. Mutations in this gene cause Griscelli syndrome type-1 (GS1), Griscelli syndrome type-3 (GS3) and neuroectodermal melanolysosomal disease, or Elejalde disease. Multiple alternatively spliced transcript variants encoding different isoforms have been reported, but the full-length nature of some variants has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
dilute lethal-20J protein; Dilute myosin heavy chain, non-muscle; dilute-opisthotonus; flailer; MYH12; MYO5; myosin 5a; Myosin heavy chain 12; myosin V; myosin VA (heavy chain 12, myoxin); myosin, heavy polypeptide kinase; Myosin-12; myosin-Va; Myoxin; MYR12; Unconventional myosin-Va
9630007J19Rik; AI413174; AI661011; D; d-120J; Dbv; Dilute; Dop; flail; flr; GS1; MVa; MYH12; MYO5; MYO5A; MyoVA; MYR12; Sev-1