|Tested species reactivity||Dog, Human, Mouse, Pig, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide, conjugated to KLH|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-24949 detects MYO6 from human, mouse, rat, canine, and porcine samples.
PA1-24949 has been successfully used in Western blot, and Immunocytochemistry procedures.
The PA1-24949 immunogen is a synthetic peptide, conjugated to KLH, corresponding to a portion of human MYO6.
Myosins belong to a superfamily of actin based motor proteins comprising at least 15 or more classes. There are two main groups of myosins: the conventional (class II) and the unconventional myosins. Myosin VI is a relatively abundant widespread unconventional myosin composed of an N-terminal motor domain, a light chain binding neck region, a coil-coiled region, and a highly conserved C-terminal domain. At the converter region between the catalytic head and the neck region of myosin VI, there is a characteristic linker of approximately 50 amino acids. Native myosin VI is apparently a two-headed dimer of the heavy chains with each heavy chain bound to calmodulin light chain. Myosin VI is a unique "reverse" actin motor in vitro i.e. to display motility towards the pointed (minus) ends of actin filaments, a direction opposite to all other currently known myosins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.