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Immunofluorescence analysis of MYO7A was performed using 70% confluent log phase L6 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MYO7A Rabbit Polyclonal Antibody (PA1936) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoskeletal localization. Panel e shows the control without primary antibody. The images were captured at 60X magnification.
|Tested species reactivity||Mouse, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: S(16) G Q E F D V P I G A V V K L C(31)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Western Blot (WB)||3-6 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-936 detects myosin VIIa from mouse tissues as well as recombinant protein.
PA1-936 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~220 kDa protein representing myosin VIIa from mouse testes preparations. PA1-936 detects recombinant mouse myosin VIIa overexpressed in Sf9 insect cell lysate.
PA1-936 immunizing peptide corresponds to amino acid residues 16-31 from mouse myosin VIIa. This sequence is completely conserved in mouse, porcine, and human myosin VIIa. PA1-936 immunizing peptide (Cat. # PEP-045) is available for use in neutralization and control experiments.
Myosin VIIa is a member of the myosin superfamily of actin-based motor proteins. Defects in the myosin VIIa gene are responsible for hearing impairment in shaker-1 (sh1) mice and causes Usher syndrome IB in humans. Usher syndrome associates congenital deafness, vestibular dysfunction, and retinitis pigmentosa and is the most common form of combined deafness and blindness. Structural features of myosin VIIa protein include an ATP binding N-terminal motor domain, a central region which possess five light-chain binding (IQ) motifs, and a C-terminal domain with three myosin tail homology (MyTH4) and talin-like homology regions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Expression of prestin in OHCs is reduced in Spag6 gene knockout mice.
PA1-936 was used in western blot to determine the expression of SPAG6 in cochlear hair cells and its influence on prestin
|Wang J,Li X,Zhang Z,Wang H,Li J||Neuroscience letters (592:42)||2015|
Myosin VIIa participates in opsin transport through the photoreceptor cilium.
PA1-936 was used in western blot to demonstrate that myosin VIIa function in opsin transport through the photoreceptor cilium
|Liu X,Udovichenko IP,Brown SD,Steel KP,Williams DS||The Journal of neuroscience : the official journal of the Society for Neuroscience (19:6267)||1999|
Notch signaling alters sensory or neuronal cell fate specification of inner ear stem cells.
PA1-936 was used in immunohistochemistry to investigate the role of Notch pathway in the fate determination of inner ear stem cells
|Jeon SJ,Fujioka M,Kim SC,Edge AS||The Journal of neuroscience : the official journal of the Society for Neuroscience (31:8351)||2011|
MicroRNA-183 family expression in hair cell development and requirement of microRNAs for hair cell maintenance and survival.
PA1-936 was used in immunohistochemistry to study the expression of microRNAs in the hair cells of the mouse inner ear
|Weston MD,Pierce ML,Jensen-Smith HC,Fritzsch B,Rocha-Sanchez S,Beisel KW,Soukup GA||Developmental dynamics : an official publication of the American Association of Anatomists (240:808)||2011|
Expression of candidate markers for stem/progenitor cells in the inner ears of developing and adult GFAP and nestin promoter-GFP transgenic mice.
PA1-936 was used in immunohistochemistry to study the mechanism for permanent sensori-neural hearing loss
|Smeti I,Savary E,Capelle V,Hugnot JP,Uziel A,Zine A||Gene expression patterns : GEP (11:22)||2011|
Phosphatase and tensin homolog deleted on chromosome 10 regulates sensory cell proliferation and differentiation of hair bundles in the mammalian cochlea.
PA1-936 was used in immunohistochemistry to investigate the effect of PTEN on auditory sensory epithelial cells
|Dong Y,Sui L,Yamaguchi F,Kamitori K,Hirata Y,Hossain MA,Suzuki A,Holley MC,Tokuda M||Neuroscience (170:1304)||2010|
Role of phosphatase and tensin homolog in the development of the mammalian auditory system.
PA1-936 was used in immunohistochemistry to investigate the involvement of PTEN in the development of mammalian auditory system
|Dong Y,Sui L,Yamaguchi F,Kamitori K,Hirata Y,Suzuki A,Holley M,Tokuda M||Neuroreport (21:731)||2010|
A missense mutation in the previously undescribed gene Tmhs underlies deafness in hurry-scurry (hscy) mice.
PA1-936 was used in immunohistochemistry to identify a novel gene Tmhs related to the formation of hair cell stereocilia.
|Longo-Guess CM,Gagnon LH,Cook SA,Wu J,Zheng QY,Johnson KR||Proceedings of the National Academy of Sciences of the United States of America (102:7894)||2005|
Retention of progenitor cell phenotype in otospheres from guinea pig and mouse cochlea.
PA1-936 was used in immunocytochemistry to investigate the progenitor cell phenotype in otosphere suspension cultures of dissociated organ of Corti from neonatal mouse and guinea pig
|Oiticica J,Barboza-Junior LC,Batissoco AC,Lezirovitz K,Mingroni-Netto RC,Haddad LA,Bento RF||Journal of translational medicine (8:null)||2010|
motor protein; MyO VIIa; myosin VIIA (Usher syndrome 1B (autosomal recessive, severe)); myosin-VIIa; shaker 1; unconventional myosin-VIIa
Hdb; Myo7; Myo7a; nmf371; polka; sh-1; sh1; USH1B