Western blot of Myosin 9B in whole cell extract of rat embryonic brain using a Myosin 9B polyclonal antibody (Product # PA1-24950) at a dilution of 1:1000 (Lane A) and 1:1000 + 20 mg/mL Myosin IXb immunizing peptide (Lane B). The antibody was developed with a Goat anti-Rabbit IgG Peroxidase and chemiluminescent substrate.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide, conjugated to KLH, corresponding to residues 1960-1980 of rat MYO9B.|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-24950 detects MYO9B from rat samples. PA1-24950 is expected to cross react with mouse and human due to sequence homology.
PA1-24950 has been successfully used in Western blot procedures.
The PA1-24950 immunogen is a synthetic peptide, conjugated to KLH, corresponding to residues 1960-1980 of rat MYO9B.
displays ATP-dependent binding to actin: binds members of the rho family of GTPases; may play a role in formation of the actin cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.