|Tested species reactivity||Mouse, Rat, Non-human primate, Dog, Human, Rabbit|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Myosin isolated from rabbit skeletal muscle.|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for immunohistochemical applications for this product is skeletal muscle. This product does not require protein digestion pre-treatment of paraffin embedded sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections.
Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Dynamic intersection of the longitudinal muscle and external anal sphincter in the layered structure of the anal canal posterior wall.
MA1-82136 was used in immunohistochemistry to study the detailed structure of the posterior anal canal
|Muro S,Yamaguchi K,Nakajima Y,Watanabe K,Harada M,Nimura A,Akita K||Surgical and radiologic anatomy : SRA (36:551)||2014|
Ex vivo differentiation of human adult bone marrow stem cells into cardiomyocyte-like cells.
MA1-82136 was used in immunocytochemistry to study the ex vivo differentiation of human adult bone marrow stem cells into cardiomyocyte-like cells
|Shim WS,Jiang S,Wong P,Tan J,Chua YL,Tan YS,Sin YK,Lim CH,Chua T,Teh M,Liu TC,Sim E||Biochemical and biophysical research communications (324:481)||2004|