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Invitrogen
Myosin Skeletal Muscle Monoclonal Antibody (414)
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Myosin Skeletal Muscle Antibody (MA1-35718) in WB
Western blot was performed using Myosin Skeletal Muscle Monoclonal Antibody (414) (Product # MA1-35718) and a 223 kDa band corresponding to Myosin Skeletal Muscle was observed in RD, C2C12 differentiated to myotubes and L6 but not in HeLa, A549, MCF7 and PC12. Whole cell extracts (30 µg lysate) of RD (Lane 1), HeLa (Lane 2), A549 (Lane 3), MCF7 (Lane 4), C2C12 (Lane 5), C2C12 differentiated to myotubes (Lan... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-35718
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken,
Human,
Mouse,
Rabbit
Published species
Rat
Host/Isotype
Mouse
/ IgM
Class
Monoclonal
Type
Antibody
Clone
414
Immunogen
Chicken skeletal muscle cell preparation
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
tissue culture supernatant
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Mab 414 reacts with myosin-containing elements in most cell types. Antigen localization: cytoplasm In Western blot experiments the antibody reacts with a 75kD part of the heavy chain (epitope location on a myosin-common sitechain), no reaction with light chains. It is not reactive with alpha-cardiac myosin. Positive control: muscle, myofibrils (chicken)
Target Information
Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: epididymis luminal protein 71; fast 2a myosin heavy chain; inclusion body myopathy 3, autosomal dominant; MGC133384; myHC-2b; myHC-2x; myHC-IIx/d; myosin heavy chain 1; myosin heavy chain 2; myosin heavy chain 2A; myosin heavy chain 2b; myosin heavy chain 2X; myosin heavy chain 4; myosin heavy chain IIa; myosin heavy chain IIb; myosin heavy chain IIx/d; Myosin heavy chain, skeletal muscle; myosin heavy chain, skeletal muscle, adult; myosin heavy chain, skeletal muscle, adult 1; myosin heavy chain, skeletal muscle, adult 2; myosin heavy chain, skeletal muscle, fetal; myosin heavy chain, skeletal muscle, juvenile; myosin, heavy chain 1, skeletal muscle, adult; myosin, heavy chain 2, skeletal muscle, adult; myosin, heavy chain 4, skeletal muscle; myosin, heavy polypeptide 1, skeletal muscle, adult; myosin, heavy polypeptide 2, skeletal muscle, adult; myosin, heavy polypeptide 4, skeletal muscle; myosin-4; skeletal muscle myosin heavy chain II-a; type IIA myosin heavy chain; unnamed protein product
Gene Aliases: A530084A17Rik; CMYO6; CMYP6; HEL71; IBM3; IId; IId/x; MdMs; MHC; MHC-2X/D; MHC2A; MHC2B; MHC2X/D; Minimsc; Minmus; MM; MYH-2B; MYH1; MYH2A; MYH2B; MYHa; MYHas8; MyHC-2A; MyHC-2B; MyHC-2x; MyHC-2X/D; MyHC-IIa; MyHC-IIb; MyHC-IId/x; MYHC-IIX; MyHC-IIx/d; Myhs-f; Myhs-f1; Myhs-f2; MYHSA1; MYHSA2; Myhsf; Myhsf1; Myhsf2
UniProt ID: (Human) P12882, (Human) Q9UKX2, (Human) Q9Y623, (Rabbit) Q28641, (Mouse) Q5SX40, (Mouse) G3UW82, (Mouse) Q5SX39
Entrez Gene ID: (Human) 4619, (Chicken) 427788, (Human) 4620, (Human) 4622, (Rabbit) 100009548, (Rabbit) 100125991, (Mouse) 17879, (Mouse) 17882, (Mouse) 17884
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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