Western blot analysis of N-Myc using anti-N-Myc monoclonal antibody (MA5-17258) on (Lane 1) HeLa Cell lysate, (Lane 2) Ramos Cell lysate, (Lane 3) HuT78 Cell lysate, (Lane 4) MCF7 Cell lysate, (Lane 5) Mouse Brain Tissue, (Lane 6) Mouse Testis Tissue, (Lane 7) Rat Brain Tissue and (Lane 8) Rat Testis Tissue.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Storage buffer||HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.03% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for this product is HeLa cell lysate.
The N-myc gene belongs to a family of proto-oncogenes, including the highly related c-myc, N-myc, and L-myc genes, that encode transcription factors sharing many structural and biochemical functions. The structural motifs conserved between the MYC proteins include a basic helix-loop-helix domain and a leucine zipper domain involved in DNA-protein and protein-protein interactions, respectively. These motifs are also found in numerous transcription factors that control cell determination during diverse cell processes such as myogenesis, neurogenesis, and sex determination.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.