Immunofluorescent analysis of N-cadherin (green) in SH-SY5Y cells. Methanol-fixed cells were blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with an N-cadherin monoclonal antibody (Product # MA1-159) at a dilution of 1:100 (right panel) or incubated in blocking buffer as a negative control (left panel) for 1 hour at room temperature, washed with PBS and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||MBP fusion protein containing the full cytoplasmic domain of human N-cadherin|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:200-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Paraffin) (IHC (P))||See 2 publications below|
N-cadherin is a calcium-dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail with a predicted molecular weight of ~90 kDa. N-cadherin is required during gastrulation for proper establishment of the left-right axis. Full length and soluble N-cadherin have been shown to promote cell-matrix adhesion and neurite outgrowth. It has been reported that aberrant expression of N-cadherin by cancer cells can contribute to invasiveness and metastasis by making the cells more motile.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Effect of N-cadherin misexpression by the mammary epithelium in mice.
MA1-159 was used in immunohistochemistry - paraffin section to investigate the effect of N-cadherin misexpression in mice
|Knudsen KA,Sauer C,Johnson KR,Wheelock MJ||Journal of cellular biochemistry (95:1093)||2005|
P-cadherin expression in breast carcinoma indicates poor survival.
MA1-159 was used in immunohistochemistry - paraffin section to discuss the role of cadherins in tumor development.
|Peralta Soler A,Knudsen KA,Salazar H,Han AC,Keshgegian AA||Cancer (86:1263)||1999|