Invitrogen

N-cadherin Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1 Published Figure

6 References

View all (57) N-cadherin antibodies

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Protocols

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Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

N-cadherin Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of N-cadherin was performed using 90% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with N-cadherin Monoclonal Antibody (Product # PA5-19486) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

N-cadherin Antibody in Immunohistochemistry (IHC) — Immunohistochemical (formalin-fixed, paraffin-embedded) staining of Human Liver Cancer tissue using Product # PA5-19486, anti-N Cadherin antibody. Primary antibody was used at a concentration of 1 µg/mL and exposed for 15 mins at room temp. The sample was pretreated using heat mediated antigen retrieval with Sodium Citrate Buffer (pH6/20mins). The detection method was a HRP conjugated polymer, DAB chromogen and the sample was counterstained with haematoxylin and mounted with DPX.

N-cadherin Antibody in Western Blot (WB) — Western blot analysis of N-cadherin was performed by loading human fetal astrocyte cell lysate (Lane 1) and 2.5 µg of human brain tissue extract (Lane 2) per well onto an SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked for 1 hour at room temperature. The membrane was probed with an N-cadherin polyclonal antibody (Product # PA5-19486) at a concentration for 1 µg/mL overnight at 4°C, washed in PBST, and probed with an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:10,000 for 1 hour at room temperature Detection was performed using ECL substrate (Product # 32106). Data courtesy of the Innovators Program.

N-cadherin Antibody — Antibody specificity was demonstrated by showing that antibodies raised against the same target protein perform similarly. Immunofluorescence of N-cadherin using N-cadherin Polyclonal Antibody (Product # PA5-19486) performed along with another N-cadherin antibody (Product # MA1-91128) shows similar localization pattern in SH-SY5Y cells. {IAV}

Product Details

PA5-19486

Applications	Tested Dilution	Publications
Western Blot (WB)	1 µg/mL	View 2 publications 2 publications
Immunohistochemistry (IHC)	-	View 3 publications 3 publications
Immunohistochemistry (Paraffin) (IHC (P))	1 µg/mL	-
Immunocytochemistry (ICC/IF)	5 µg/mL	View 1 publication 1 publication
Immunoprecipitation (IP)	Assay-Dependent	-

Product Specifications
Species Reactivity	Human, Mouse, Pig, Rat, Xenopus
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide conjugated to KLH derived from within residues 800 - 900 of Human N Cadherin. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.4
Contains	0.02% sodium azide
Storage conditions	-20°C or -80°C if preferred
RRID	AB_10979609

Product Specific Information

Heat mediated antigen retrieval recommended prior to tissue staining.

This antibody is predicted to react with chicken and cow based on sequence homology.

Target Information

N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Neonatal Hyperoxia Downregulates Claudin-4, Occludin, and ZO-1 Expression in Rat Kidney Accompanied by Impaired Proximal Tubular Development.

Oxidative medicine and cellular longevity

Xu X,Zhang X,Gao L,Liu C,You K

PA5-19486 was used in Immunohistochemistry-immunofluorescence to investigate how neonatal hyperoxia affected the expression of key tight junction proteins and inflammatory factors (IL-6 and TNF-α) in the developing rat kidneys and elucidated their correlation with renal injury.

Tue Sep 07 00:00:00 EDT 2021

H19-Dependent Transcriptional Regulation of β3 and β4 Integrins Upon Estrogen and Hypoxia Favors Metastatic Potential in Prostate Cancer.

International journal of molecular sciences

Bacci L,Aiello A,Ripoli C,Loria R,Pugliese D,Pierconti F,Rotili D,Strigari L,Pinto F,Bassi PF,Mai A,Grassi C,Pontecorvi A,Falcioni R,Farsetti A,Nanni S

PA5-19486 was used in Western Blot to dissect the contribution of long non-coding RNA H19 to PCa metastatic potential under combined stimuli.

Sat Aug 17 00:00:00 EDT 2019

VCAM-1 induces signals that stimulate ZO-1 serine phosphorylation and reduces ZO-1 localization at lung endothelial cell junctions.

Journal of leukocyte biology

Abdala-Valencia H,Kountz TS,Marchese ME,Cook-Mills JM

PA5-19486 was used in Immunohistochemistry to demonstrate that during allergic lung response there was reduced zonula occludens-1 localisation in lung epithelial cell juncyions whereas there was increases lung endothelial cell expression of VCAM-1, N-cadherin and angiomotin.

Sun Jul 01 00:00:00 EDT 2018

ZEB1 Regulates Multiple Oncogenic Components Involved in Uveal Melanoma Progression.

Scientific reports

Chen Y,Lu X,Montoya-Durango DE,Liu YH,Dean KC,Darling DS,Kaplan HJ,Dean DC,Gao L,Liu Y

PA5-19486 was used in Immunocytochemistry-immunoflourescence to study the role of ZEB1 in human uveal melanoma progression.

Fri Mar 03 00:00:00 EST 2017

Cisplatin promotes mesenchymal-like characteristics in osteosarcoma through Snail.

Oncology letters

Fang S,Yu L,Mei H,Yang J,Gao T,Cheng A,Guo W,Xia K,Liu G

PA5-19486 was used in immunocytochemistry and western blot to test if chemotherapy affects the epithelial-mesenchymal transition of osteosarcoma cells

Thu Dec 01 00:00:00 EST 2016

Cisplatin promotes mesenchymal-like characteristics in osteosarcoma through Snail.

Oncology letters

Fang S,Yu L,Mei H,Yang J,Gao T,Cheng A,Guo W,Xia K,Liu G

PA5-19486 was used in immunocytochemistry and western blot to test if chemotherapy affects the epithelial-mesenchymal transition of osteosarcoma cells

Thu Dec 01 00:00:00 EST 2016

Bioinformatics

Protein Aliases: cadherin 2 type 1 N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal) L homeolog; cadherin 2, type 1, N-cadherin a (neuronal); Cadherin-2; Cadherin-2A; Cadherin2; calcium-dependent adhesion protein, neuronal; CD325; CDw325; CDw325 antigen; N-cadherin; N-cadherin 1; N-cadherin A; N-cadherin-2; Neural cadherin; Neural cadherin A; Neural cadherin-2

Gene Aliases: cadherin-2; CD325; CDH2; cdh2-a; cdh2-b; cdh2a; CDHN; cdhn-a; CDw325; N-Cad; N-cadherin; NCAD; XELAEV_18031953mg

UniProt ID: (Human) P19022, (Mouse) P15116, (Rat) Q9Z1Y3, (Xenopus) P33147

Entrez Gene ID: (Pig) 100515322, (Human) 1000, (Mouse) 12558, (Rat) 83501, (Xenopus) 378518

Molecular Function: cadherin