Invitrogen
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1 µg/mL | View 2 publications 2 publications |
Immunohistochemistry (IHC) |
- | View 3 publications 3 publications |
Immunohistochemistry (Paraffin) (IHC (P)) |
1 µg/mL | - |
Immunocytochemistry (ICC/IF) |
5 µg/mL | View 1 publication 1 publication |
Immunoprecipitation (IP) |
Assay-Dependent | - |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse, Pig, Rat, Xenopus |
Published species |
Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Synthetic peptide conjugated to KLH derived from within residues 800 - 900 of Human N Cadherin. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.4 |
Contains |
0.02% sodium azide |
Storage conditions |
-20°C or -80°C if preferred |
RRID |
AB_10979609 |
Heat mediated antigen retrieval recommended prior to tissue staining.
This antibody is predicted to react with chicken and cow based on sequence homology.
N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: cadherin 2 type 1 N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal) L homeolog; cadherin 2, type 1, N-cadherin a (neuronal); Cadherin-2; Cadherin-2A; Cadherin2; calcium-dependent adhesion protein, neuronal; CD325; CDw325; CDw325 antigen; N-cadherin; N-cadherin 1; N-cadherin A; N-cadherin-2; Neural cadherin; Neural cadherin A; Neural cadherin-2
Gene Aliases: cadherin-2; CD325; CDH2; cdh2-a; cdh2-b; cdh2a; CDHN; cdhn-a; CDw325; N-Cad; N-cadherin; NCAD; XELAEV_18031953mg
UniProt ID: (Human) P19022, (Mouse) P15116, (Rat) Q9Z1Y3, (Xenopus) P33147
Entrez Gene ID: (Pig) 100515322, (Human) 1000, (Mouse) 12558, (Rat) 83501, (Xenopus) 378518
Molecular Function:
cadherin
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