Invitrogen

N-cadherin Polyclonal Antibody

This Antibody was verified by Independent antibody to ensure that the antibody binds to the antigen stated. View Details

1 Published Figure

2 References

View all (47) N-cadherin antibodies

Datasheet

Datasheet

Product Image Gallery

N-cadherin Antibody — Figure 2. Cisplatin promotes EMT-TFs in osteosarcoma. (A) The relative expression of EMT-TFs, including Snail/Slug and Zeb1/2 were observed to be significantly upregulated in the cisplatin treated cells by quantitative polymerase chain reaction. **P<0.01. (B) Expression of Snail and Slug was analyzed by western blotting and cells treated with cisplatin demonstrated increased expression. (C) Snail and N-cadherin expression was increased following cisplatin exposure xenograft assay in vivo (magnification, x200; Scale, 50 um). EMT, epithelial-mesenchymal transition; TF, transcription factor; Zeb, zinc finger E-box binding homeobox.

N-cadherin Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of N-cadherin was performed using 90% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with N-cadherin Monoclonal Antibody (Product # PA5-19486) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

N-cadherin Antibody in Immunohistochemistry (IHC) — Immunohistochemical (formalin-fixed, paraffin-embedded) staining of Human Liver Cancer tissue using Product # PA5-19486, anti-N Cadherin antibody. Primary antibody was used at a concentration of 1 µg/mL and exposed for 15 mins at room temp. The sample was pretreated using heat mediated antigen retrieval with Sodium Citrate Buffer (pH6/20mins). The detection method was a HRP conjugated polymer, DAB chromogen and the sample was counterstained with haematoxylin and mounted with DPX.

N-cadherin Antibody in Western Blot (WB) — Western blot analysis of N-cadherin was performed by loading human fetal astrocyte cell lysate (Lane 1) and 2.5 µg of human brain tissue extract (Lane 2) per well onto an SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked for 1 hour at room temperature. The membrane was probed with an N-cadherin polyclonal antibody (Product # PA5-19486) at a concentration for 1 µg/mL overnight at 4°C, washed in PBST, and probed with an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:10,000 for 1 hour at room temperature Detection was performed using ECL substrate (Product # 32106). Data courtesy of the Innovators Program.

N-cadherin Antibody in Independent antibody — Antibody specificity was demonstrated by showing that antibodies raised against the same target protein perform similarly. Immunofluorescence of N-cadherin using N-cadherin Polyclonal Antibody (Product # PA5-19486) performed along with another N-cadherin antibody (Product # MA1-191128) shows similar localization pattern in SH-SY5Y cells. {IAV}

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

10 µg/mL

Immunocytochemistry (ICC)

5 µg/mL

Immunofluorescence (IF)

5 µg/mL

Immunohistochemistry (Frozen) (IHC (F))

1:300

Immunohistochemistry (Paraffin) (IHC (P))

1 µg/mL

Immunoprecipitation (IP)

Assay-Dependent

Western Blot (WB)

1 µg/mL

Published Applications

Western Blot (WB)

See 1 publication below

Immunohistochemistry (IHC)

See 1 publication below

Product Specifications

Species Reactivity

Human, Mouse, Pig, Rat, Xenopus

Published species

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 800 - 900 of Human N Cadherin.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

RRID

AB_10979609

Product Specific Information

Heat mediated antigen retrieval recommended prior to tissue staining.

This antibody is predicted to react with chicken and cow based on sequence homology.

Target Information

N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: cadherin 2 type 1 N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal) L homeolog; cadherin 2, type 1, N-cadherin a (neuronal); Cadherin-2; Cadherin-2A; calcium-dependent adhesion protein, neuronal; CD325; CDHN; CDw325; CDw325 antigen; N-cadherin; N-cadherin 1; N-cadherin A; N-cadherin-2; NCAD; Neural cadherin; Neural cadherin A; Neural cadherin-2

Gene Aliases: cadherin-2; CD325; CDH2; cdh2-a; cdh2-b; cdh2a; CDHN; cdhn-a; CDw325; N-Cad; N-cadherin; NCAD; XELAEV_18031953mg

UniProt ID: (Human) P19022, (Mouse) P15116, (Rat) Q9Z1Y3, (Xenopus) P33147

Entrez Gene ID: (Human) 1000, (Mouse) 12558, (Rat) 83501, (Xenopus) 378518

Molecular Function: cadherin