Invitrogen

N-cadherin Polyclonal Antibody

Product Image Gallery

N-cadherin Antibody in Immunocytochemistry (ICC) — Immunocytochemical analysis of N-cadherin in mouse embryonic stem cells using a N-cadherin Polyclonal antibody (Product # PA5-85916) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

N-cadherin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of N-cadherin of paraffin-embedded Human liver carcinoma tissue using a N-cadherin Polyclonal antibody (Product #PA5-85916). Counter stained with hematoxylin.

N-cadherin Antibody in Western Blot (WB) — Western blot analysis of N-cadherin in different lysates using a Polyclonal antibody (Product #PA5-85916) at a dilution of 1:500. Positive control: Lane 1: Mouse heart Lane2 : Human heart.

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

1:50

Immunocytochemistry (ICC)

1:200

Immunofluorescence (IF)

1:200

Immunohistochemistry (IHC)

1:200

Western Blot (WB)

1:500

Product Specifications

Species Reactivity

Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to Human N-Cadherin aa 400-430

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.4, with 1% BSA, 40% Glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Target Information

N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; calcium-dependent adhesion protein, neuronal; CD325; CDw325; N-cadherin; N-cadherin 1; Neural cadherin

Gene Aliases: CD325; CDH2; CDHN; CDw325; N-cadherin; NCAD

UniProt ID: (Human) P19022, (Mouse) P15116

Entrez Gene ID: (Human) 1000, (Mouse) 12558

Molecular Function: cadherin