Invitrogen
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:1,000 | View 4 publications 4 publications |
Immunohistochemistry (IHC) |
- | View 2 publications 2 publications |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:20 | - |
Immunocytochemistry (ICC/IF) |
1:50-1:200 | - |
Immunoprecipitation (IP) |
5 µg | - |
Product Specifications | |
---|---|
Species Reactivity |
Hamster, Human, Mouse, Rabbit |
Published species |
Avian, Human |
Host/Isotype |
Mouse / IgG1 |
Class |
Monoclonal |
Type |
Antibody |
Clone |
8C11 |
Immunogen |
Bacterial fusion protein corresponding to the extracellular domain of N-cadherin. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Protein G |
Storage buffer |
PBS with 1mg/mL BSA |
Contains |
0.05% sodium azide |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
RRID |
AB_326076 |
MA1-2002 detects N-Cadherin from human neuronal samples, including SHSY5Y cells. MA1-2002 also detects N-Cadherin in rabbit and hamster brain lysates. MA1-2002 does not detect N-Cadherin in lysates from mouse, rat, pig and cow brain. This antibody is not suitable for mouse C2C12 cell lysates in Western blot analysis.
MA1-2002 has been successfully used in Western blot, Immunoprecipitation, IHC (P) and Immunofluorescence applications. By Western blot, this antibody detects N-Cadherin at 130 kDa. This antibody is not suitable for frozen tissues in immunohistochemistry applications.
The MA1-2002 immunogen is a bacterial fusion protein to the extracellular domain of N-cadherin (a domain between EC3 and EC4, amino acid residues 92-593 from human N-cadherin).
N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: cadherin 2, type 1 preproprotein; cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; Cadherin2; calcium-dependent adhesion protein, neuronal; CD325; CDw325; CDw325 antigen; N-cadherin; N-cadherin 1; Neural cadherin
Gene Aliases: CD325; CDH2; CDHN; CDw325; I79_014914; N-cadherin; NCAD
UniProt ID: (Human) P19022, (Mouse) P15116
Entrez Gene ID: (Human) 1000, (Rabbit) 100344571, (Mouse) 12558, (Chinese hamster) 100689204
Molecular Function:
cadherin
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