Immunofluorescent analysis of N-Cadherin in SHSY5Y cells (human neuroblast). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed without (left panel) or with (right panel) an N-Cadherin monoclonal antibody (Product # MA1-2002) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody (Product # 35502). F-Actin (red) was stained with DyLight-554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight at 20X magnification.
|Tested species reactivity||Hamster, Human, Mouse, Rabbit|
|Published species reactivity||Avian, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Bacterial fusion protein corresponding to the extracellular domain of N-cadherin.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1:50 - 1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Immunoprecipitation (IP)||5 µg|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-2002 detects N-Cadherin from human neuronal samples, including SHSY5Y cells. MA1-2002 also detects N-Cadherin in rabbit and hamster brain lysates. MA1-2002 does not detect N-Cadherin in lysates from mouse, rat, pig and cow brain. This antibody is not suitable for mouse C2C12 cell lysates in Western blot analysis.
MA1-2002 has been successfully used in Western blot, Immunoprecipitation, IHC (P) and Immunofluorescence applications. By Western blot, this antibody detects N-Cadherin at 130 kDa. This antibody is not suitable for frozen tissues in immunohistochemistry applications.
The MA1-2002 immunogen is a bacterial fusion protein to the extracellular domain of N-cadherin (a domain between EC3 and EC4, amino acid residues 92-593 from human N-cadherin).
The cadherin superfamily of proteins are a group of calcium mediated cell-cell adhesion molecules. Cadherins are responsible for a whole range of processes including development, wound healing, cell-cell signaling, cell growth and differentiation. N-cadherin is found in many locations including cardiac adherins junctions, oral squamous epithelial cells, and breast epithelial cells. Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Control of cell-cell forces and collective cell dynamics by the intercellular adhesome.
MA1-2002 was used in immunohistochemistry to elucidate the contributions of cell-cell adhesion proteins to intercellular force and epithelial tissue dynamics.
|Bazellières E,Conte V,Elosegui-Artola A,Serra-Picamal X,Bintanel-Morcillo M,Roca-Cusachs P,Muñoz JJ,Sales-Pardo M,Guimerà R,Trepat X||Nature cell biology (17:409)||2015|
Distinct PTPmu-associated signaling molecules differentially regulate neurite outgrowth on E-, N-, and R-cadherin.
MA1-2002 was used in western blot to investigate the effects of PTPmu on cadherin-dependent neurite outgrowth
|Oblander SA,Brady-Kalnay SM||Molecular and cellular neurosciences (44:78)||2010|
N-cadherin-catenin complexes form prior to cleavage of the proregion and transport to the plasma membrane.
MA1-2002 was used in western blot to investigate N-cadherin processing by proteolysis and the formation of N-cadherin-catenin complexes
|Wahl JK,Kim YJ,Cullen JM,Johnson KR,Wheelock MJ||The Journal of biological chemistry (278:17269)||2003|