|Tested species reactivity||Bovine, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues R(808) M D E R P I H A E P Q Y P V R S A A P(827) of human N-Cadherin.|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||5-10 µg/ml|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||5-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-20359 detects N-Cadherin in human, mouse, and bovine samples. This antibody does not cross-react with E or P Cadherin.
PA1-20359 has been successfully used in immunohistochemistry (frozen and FFPE) and Western blot procedures.
The PA1-20359 immunogen is a synthetic peptide corresponding to residues R(808) M D E R P I H A E P Q Y P V R S A A P(827) of human N-Cadherin.
This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.
IP-MS enrichment of CDH2 (LFQ intensity): CDH2 was enriched 16-fold from A549 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and CDH2 antibody (Part No. PA1-20359). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.