Immunohistochemical (formalin-fixed, paraffin-embedded) staining of Human Liver Cancer tissue using PA5-19486, anti-N Cadherin antibody. Primary antibody was used at a concentration of 1 ug/ml and exposed for 15 mins at room temp. The sample was pretreated using heat mediated antigen retrieval with Sodium Citrate Buffer (pH6 / 20mins). The detection method was a HRP conjugated polymer, DAB chromogen and the sample was counterstained with haematoxylin and mounted with DPX.
|Tested species reactivity||Human, Mouse, Pig, Rat, Xenopus|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 800 - 900 of Human N Cadherin.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||10 µg/ml|
|Immunofluorescence (IF)||5 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||1:300|
|Immunohistochemistry (Paraffin) (IHC (P))||1 µg/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
Heat mediated antigen retrieval recommended prior to tissue staining.
This antibody is predicted to react with chicken and cow based on sequence homology.
This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cisplatin promotes mesenchymal-like characteristics in osteosarcoma through Snail.
PA5-19486 was used in immunocytochemistry and western blot to test if chemotherapy affects the epithelial-mesenchymal transition of osteosarcoma cells
|Fang S,Yu L,Mei H,Yang J,Gao T,Cheng A,Guo W,Xia K,Liu G||Oncology letters (12:5007)||2016|