|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||The complete coding region of the human p95/NBS1 expressed in E. coli.|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1-2 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-23265 detects NBS1 in Human samples.
MA1-23265 has been successfully used in Immunoprecipitation and Western Blot procedures. Predicted molecular weight 91 kDa. Positive control: MCF7, HeLa (NE), Raji.
MA1-23265 immunogen corresponds to The complete coding region of the human p95/NBS1 expressed in E. coli.
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
Nijmegen breakage syndrome (NBS, nibrin) is an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The 1C3 antibody recognizes the 95 kDa NBS protein which contains a fork FHA head-associated domain that is adjacent to BRCT, a breast cancer C terminal domain, that has been implicated in protein-protein interactions. NBS is member of the Mre11/Rad50, p400 double-strand break repair complex involved in double-strand break repair and cell-cycle checkpoint functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.