Immunohistochemistry analysis of NCAM1 in 4% PFA, OCT embedded frozen mouse E16.5 lung sections. Sections were blocked and probed with a NCAM1 mouse monoclonal antibody (Product # MA5-14140) at a dilution of 1:1000. Detection was performed using an Alexa Fluor-568-conjugated anti-mouse secondary antibody (A11004).
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Pig, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Homogenous suspension of 16 week human fetal brain|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Immunoprecipitation (IP)||2 µg/mg protein lysate|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14140 targets L1 Cell Adhesion Molecule in immunohistochemistry (paraffin), immunoprecipitation, and Western blot applications and shows reactivity with human samples.
The MA5-14140 immunogen is homogenous suspension of 16 week human fetal brain.
MA5-14140 has been successfully used in immunohistochemistry analysis of NCAM1 in mouse E16.5 frozen lung sections.
The L1CAM gene, which is located in Xq28, is involved in three distinct conditions: 1) HSAS (hydrocephalus-stenosis of the aqueduct of Sylvius); 2) MASA (mental retardation, aphasia, shuffling gait, adductus thumbs); and 3) SPG1 (spastic paraplegia). The L1, neural cell adhesion molecule (L1CAM) also plays an important role in axon growth, fasciculation, neural migration and in mediating neuronal differentiation. Expression of L1 protein is restricted to tissues arising from neuroectoderm.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Targeting L1 cell adhesion molecule expression using liposome-encapsulated siRNA suppresses prostate cancer bone metastasis and growth.
MA5-14140 was used in immunohistochemistry to determine how using liposome-encapsulated siRNA to target L1 cell adhesion molecule expression suppresses prostate cancer bone metastasis and growth
|Sung SY,Wu IH,Chuang PH,Petros JA,Wu HC,Zeng HJ,Huang WC,Chung LW,Hsieh CL||Oncotarget (5:9911)||2014|
Inflammatory responses in the muscle coat of stomach and small bowel in the postoperative ileus model of guinea pig.
MA5-14140 was used in immunohistochemistry to use a guinea pig model of postoperative ileus to study the muscle coat inflammatory responses of the stomach and small intestine
|Choi HK,Lee YJ,Lee YH,Park JP,Min K,Park H||Yonsei medical journal (54:1336)||2013|
Adhesion molecule L1 is down-regulated in malignant peripheral nerve sheath tumors versus benign neurofibromatosis type 1-associated tumors.
MA5-14140 was used in immunohistochemistry to study the expression of the L1 adhesion molecule in malignant peripheral nerve sheath tumors and benign neurofibromatosis type 1-associated tumors
|Blessmann M,Gröbe A,Quaas A,Kaifi JT,Mistakidis G,Bernreuther C,Sauter G,Gros S,Rawnaq T,Friedrich R,Mautner VF,Smeets R,Heiland M,Schachner M,Izbicki JR||Oral surgery, oral medicine, oral pathology and oral radiology (113:239)||2012|
DOG1 and CD117 are the antibodies of choice in the diagnosis of gastrointestinal stromal tumours.
MA5-14140 was used in immunohistochemistry to study the value of COG1 and CD117 immunohistochemistry in the diagnosis of gastrointestinal stromal tumors
|Novelli M,Rossi S,Rodriguez-Justo M,Taniere P,Seddon B,Toffolatti L,Sartor C,Hogendoorn PC,Sciot R,Van Glabbeke M,Verweij J,Blay JY,Hohenberger P,Flanagan A,Dei Tos AP||Histopathology (57:259)||2010|
Subset of esophageal adenocarcinoma expresses adhesion molecule l1 in contrast to squamous cell carcinoma.
MA5-14140 was used in immunohistochemistry to study neural cell adhesion molecule L1 overexpression in a subgroup of esophageal cancer
|Rawnaq T,Kleinhans H,Uto M,Schurr PG,Reichelt U,Cataldegirmen G,Gawad KA,Yekebas EF,Schachner M,Izbicki JR,Kaifi JT||Anticancer research (29:1195)||2009|
L1 is associated with favorable outcome in neuroblastomas in contrast to adult tumors.
MA5-14140 was used in immunohistochemistry to study the association of the L1 neuronal adhesion molecule with favorable outcome in childhood neuroblastomas
|Wachowiak R,Fiegel HC,Kaifi JT,Quaas A,Krickhahn A,Schurr PG,Erttmann R,Schachner M,Kluth D,Sauter G,Izbicki JR||Annals of surgical oncology (14:3575)||2007|
L1 is associated with micrometastatic spread and poor outcome in colorectal cancer.
MA5-14140 was used in immunohistochemistry to study the role of the L1 adhesion molecule in micrometastatic spread and poor outcome in colorectal cancer
|Kaifi JT,Reichelt U,Quaas A,Schurr PG,Wachowiak R,Yekebas EF,Strate T,Schneider C,Pantel K,Schachner M,Sauter G,Izbicki JR||Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc (20:1183)||2007|
L1 (CD171) is highly expressed in gastrointestinal stromal tumors.
MA5-14140 was used in immunohistochemistry to study the expression of L1 (CD171) in gastrointestinal stromal tumors
|Kaifi JT,Strelow A,Schurr PG,Reichelt U,Yekebas EF,Wachowiak R,Quaas A,Strate T,Schaefer H,Sauter G,Schachner M,Izbicki JR||Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc (19:399)||2006|
Characterization of gene expression in mucinous cystic neoplasms of the pancreas using oligonucleotide microarrays.
MA5-14140 was used in immunohistochemistry to investigate the changes of gene expression in pancreatic mucinous cystic neoplasms
|Fukushima N,Sato N,Prasad N,Leach SD,Hruban RH,Goggins M||Oncogene (23:9042)||2004|
Two alcohol binding residues interact across a domain interface of the L1 neural cell adhesion molecule and regulate cell adhesion.
MA5-14140 was used in flow cytometry to characterize two alcohol binding residues in L1 neural cell adhesion molecule and their involvement in cell adhesion
|Dou X,Menkari CE,Shanmugasundararaj S,Miller KW,Charness ME||The Journal of biological chemistry (286:16131)||2011|
Identification of cell surface and secreted proteins essential for tumor cell survival using a genetic suppressor element screen.
MA5-14140 was used in flow cytometry to identify molecular markers and therapeutic targets for tumors
|Gelman MS,Ye XK,Stull R,Suhy D,Jin L,Ng D,Than B,Ji M,Pan A,Perez P,Sun Y,Yeung P,Garcia LM,Harte R,Lu Y,Lamar E,Tavassoli R,Kennedy S,Osborn S,Chin DJ,Meshaw K,Holzmayer TA,Axenovich SA,Abo A||Oncogene (23:8158)||2004|
SSEA-1 is an enrichment marker for tumor-initiating cells in human glioblastoma.
MA5-14140 was used in western blot to investigate the distribution and localization of SSEA-1 in human glioblastoma multiforme cells
|Son MJ,Woolard K,Nam DH,Lee J,Fine HA||Cell stem cell (4:440)||2009|
|Not Applicable||Not Cited||
Extracellular signal-regulated kinase (ERK)-dependent gene expression contributes to L1 cell adhesion molecule-dependent motility and invasion.
MA5-14140 was used in western blot to elucidate the association between L1 expression and motile processes involved in metastasis and development
|Silletti S,Yebra M,Perez B,Cirulli V,McMahon M,Montgomery AM||The Journal of biological chemistry (279:28880)||2004|
Patterning axonal guidance molecules using a novel strategy for microcontact printing.
MA5-14140 was used in western blot to develop a protocol for microprinting proteins on a substrate in order to control neurite growth in culture
|Oliva AA,James CD,Kingman CE,Craighead HG,Banker GA||Neurochemical research (28:1639)||2003|