Invitrogen

NCBP1 Polyclonal Antibody

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NCBP1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of HeLa cells using Product # PA5-19721, anti-NCBP1 antibody. The cells were fixed with PFA (4%) for 10 minutes, permabilised with BSA (1%), normal goat serum (10%) and glycine (0.3 M) in 0.1% T-BST for 1 hour and exposed to the primary antibody at a concentration of 5 µg/mL for 1 hour at room temp. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAPI (blue).

NCBP1 Antibody in Western Blot (WB) — Western blot analysis of NCBP1 using a polyclonal antibody (Product # PA5-19721).

Product Details

PA5-19721

Applications	Tested Dilution	Publications
Western Blot (WB)	1 µg/mL	-
Immunocytochemistry (ICC/IF)	5 µg/mL	-
Immunoprecipitation (IP)	5 µg/mL	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human NCBP1. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.4, with 1% BSA
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_10987223

Product Specific Information

This antibody is predicted to react with mouse, rat, chicken, Xenopus laevis and cynomolgus monkey based on sequence homology.

Target Information

May be involved in protein trafficking from the plasma membrane to the early endosome (EE) as well as in homotypic fusion of endocytic organelles. Mediates the endocytic trafficking from early endosomes to late endosomes and lysosomes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 80 kDa nuclear cap-binding protein; CBP80; MGC2087; NCBP 80 kDa subunit; nuclear cap binding protein subunit 1, 80kD; nuclear cap binding protein subunit 1, 80kDa; Nuclear cap-binding protein subunit 1; RP11-546O6.1

Gene Aliases: CBP80; NCBP; NCBP1; Sto1

UniProt ID: (Human) Q09161

Entrez Gene ID: (Human) 4686

Molecular Function: RNA metabolism protein RNA processing factor RNA splicing factor nucleic acid metabolism protein