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|Tested species reactivity||Human, Primate|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant fragment of human NCK1 expressed in E. Coli.|
|Storage buffer||PBS with 0.5% proprietary stabilizer|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:200 - 1:400|
|Immunohistochemistry (IHC)||1:200 - 1:1000|
|Western Blot (WB)||1:500 - 1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-17132 targets NCK1 in FACS, IHC, pep-ELISA, and WB applications and shows reactivity with Human and Non-human primate samples.
The MA5-17132 immunogen is purified recombinant fragment of human NCK1 expressed in E. Coli.
MA5-17132 detects NCK1 which has a predicted molecular weight of approximately 47kDa.
The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
melanoma NCK protein; NCK tyrosine kinase; non-catalytic region of tyrosine kinase; SH2/SH3 adaptor protein NCK-alpha
NCK; nck-1; NCK1; NCKalpha