Invitrogen

NCK1 Recombinant Rabbit Monoclonal Antibody (JU41-80)

View all (11) NCK1 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

NCK1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of NCK1 in HUVEC cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with NCK1 monoclonal antibody (Product # MA5-34616), followed by DAPI (blue).

NCK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of NCK1 in paraffin-embedded mouse testis tissue. Samples were incubated with NCK1 monoclonal antibody (Product # MA5-34616), and followed by hematoxylin.

NCK1 Antibody in Western Blot (WB) — Western blot analysis of NCK1 in Lane 1: HeLa, Lane 2: NIH-3T3, Lane 3: Rat kidney tissue, Lane 4: Mouse testis tissue. Samples were incubated with NCK1 monoclonal antibody (Product # MA5-34616), at a dilution of 1:500.

NCK1 Antibody in Flow Cytometry (Flow) — Flow cytometry of NCK1 in Jurkat cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with NCK1 monoclonal antibody (Product # MA5-34616) at a dilution of 1:50, followed by Alexa Fluor 488-conjugated goat anti rabbit IgG.

Product Details

MA5-34616

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:50-1:200

Flow Cytometry (Flow)

1:50-1:100

Immunoprecipitation (IP)

1:10-1:50

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JU41-80

Immunogen

Recombinant protein within Human NCK1 aa 204-296

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles, store in dark

RRID

AB_2848524

Product Specific Information

Positive Control: NIH-3T3, Hela, rat kidney tissue lysate, mouse testis tissue lysate, A431, HUVEC, LOVO, rat testis tissue, human tonsil tissue, human kidney tissue.

Target Information

NCK1 is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cytoplasmic protein NCK1; melanoma NCK protein; MGC12668; NCK adaptor protein 1; NCK tyrosine kinase; Nck-1; non-catalytic region of tyrosine kinase; SH2/SH3 adaptor protein NCK-alpha

Gene Aliases: NCK; nck-1; NCK1; NCKalpha

UniProt ID: (Human) P16333

Entrez Gene ID: (Human) 4690

Molecular Function: scaffold/adaptor protein

Function(s)

negative regulation of protein kinase activity substrate-dependent cell migration, cell extension actin filament organization signal complex assembly vesicle-mediated transport lamellipodium assembly positive regulation of actin filament polymerization negative regulation of peptidyl-serine phosphorylation positive regulation of translation in response to endoplasmic reticulum stress Fc-gamma receptor signaling pathway involved in phagocytosis positive regulation of T cell proliferation T cell activation positive regulation of GTPase activity positive regulation of transcription from RNA polymerase II promoter vascular endothelial growth factor receptor signaling pathway T cell receptor signaling pathway response to other organism negative regulation of cell death peptidyl-serine dephosphorylation positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway positive regulation of cap-dependent translational initiation positive regulation of cap-independent translational initiation negative regulation of PERK-mediated unfolded protein response negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation negative regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress

Process(es)

protein kinase inhibitor activity SH3/SH2 adaptor activity ARF guanyl-nucleotide exchange factor activity receptor binding protein binding cytoskeletal adaptor activity protein domain specific binding receptor signaling complex scaffold activity protein binding, bridging receptor tyrosine kinase binding eukaryotic initiation factor eIF2 binding cadherin binding involved in cell-cell adhesion