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NCKAP1/NAP125 Antibody (A305-179A-T) in IP
Detection of human NCKAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NCKAP1 antibody A305-179A (lot A305-179A-1) used for IP at 6 µg per reaction. NCKAP1 was also immunoprecipitated by rabbit anti-NCKAP1 antibody A305-178A. ... View More
Product Details
A305-179A-T
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 925 to 975 of human Nck-associated protein 1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse, Rat, Zebrafish and X. laevis
Target Information
This gene interacts with Nck and may be involved in the downstream signaling of Nck and shares similarity with the mouse Dhm1 and the yeast dhp1 gene. The yeast gene is involved in homologous recombination and RNA metabolism, such as RNA synthesis and RNA trafficking. Complementation studies show that Dhm1 has a similar function in mouse as dhp1. The function of the human gene has not yet been determined. Transcript variants encoding different isoforms have been noted for this gene; however, their full-length nature is not known.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Membrane-associated protein HEM-2; NAP 1; Nck-associated protein 1; p125Nap1
Gene Aliases: HEM2; KIAA0587; NAP1; NAP125; NCKAP1; p125Nap1
UniProt ID: (Human) Q9Y2A7
Entrez Gene ID: (Human) 10787
apoptotic process
central nervous system development
positive regulation of lamellipodium assembly
viral process
Rac protein signal transduction
Fc-gamma receptor signaling pathway involved in phagocytosis
vascular endothelial growth factor receptor signaling pathway
positive regulation of Arp2/3 complex-mediated actin nucleation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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