Immunofluorescent analysis of NCoR (green) showing staining in the nucleus and cytoplasm of MCF-7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a NCoR polyclonal antibody (Product # PA1-844A) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C P(2427) A P L L S A Q Y E T L S D S D D(2443) of mouse N-CoR.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||Assay dependent|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200-1:1000|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-844A detects human and mouse nuclear receptor corepressor (N-CoR).
PA1-844A has been successfully used in Western blot, ICC/IF, IHC (P) and immunoprecipitation (ChIP) procedures. By Western blot, this antibody detects a protein at ~270 kDa representing N-CoR from HeLa cell extracts and mouse RAW 264.7/primary macrophages. This antibody is not recommended for mouse C2C12 cells in immunofluorescent applications.
The PA1-844A immunogen is a synthetic peptide corresponding to residues C P(2427) A P L L S A Q Y E T L S D S D D(2443) of mouse N-CoR. This immunizing peptide (Cat. # PEP-061) is available for use in neutralization and control experiments.
Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR) are related transcriptional corepressors which contain two distinct domains capable of interacting with unliganded nuclear receptors to repress their basal transcriptional activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
COPS5 amplification and overexpression confers tamoxifen-resistance in ER¿-positive breast cancer by degradation of NCoR.
PA1-844A was used in immunohistochemistry - paraffin section and western blot to analyze tamoxifen-resistance in ERalpha-positive breast cancer by degradation of NCoR due to COPS5 amplification and overexpression
|Lu R,Hu X,Zhou J,Sun J,Zhu AZ,Xu X,Zheng H,Gao X,Wang X,Jin H,Zhu P,Guo L||Nature communications (7:null)||2016|
CtBP2 modulates the androgen receptor to promote prostate cancer progression.
PA1-844A was used in western blot to correlate CTBP2 SNPs with prostate cancer development and AR-enhancer activity
|Takayama K,Suzuki T,Fujimura T,Urano T,Takahashi S,Homma Y,Inoue S||Cancer research (74:6542)||2014|
Activity-dependent phosphorylation of MeCP2 threonine 308 regulates interaction with NCoR.
PA1-844A was used in western blot to study an activity-dependent phosphorylation site on MeCP2 that modulates interaction with NCoR
|Ebert DH,Gabel HW,Robinson ND,Kastan NR,Hu LS,Cohen S,Navarro AJ,Lyst MJ,Ekiert R,Bird AP,Greenberg ME||Nature (499:341)||2013|
Electromagnetic fields alter the expression of estrogen receptor cofactors in breast cancer cells.
PA1-844A was used in western blot to investigate the effect of electromagnetic fields on expression of estrogen receptor cofactors in MCF-7 cells
|Girgert R,Gründker C,Emons G,Hanf V||Bioelectromagnetics (29:169)||2008|
Nuclear receptor corepressor is a novel regulator of phosphatidylinositol 3-kinase signaling.
PA1-844A was used in western blot to indicate that NCoR is a novel regulator of PI3K signaling and could modulate thyroid tumor progression
|Furuya F,Guigon CJ,Zhao L,Lu C,Hanover JA,Cheng SY||Molecular and cellular biology (27:6116)||2007|
|Not Applicable||Not Cited||
Energy homeostasis targets chromosomal reconfiguration of the human GH1 locus.
PA1-844A was used in ChIP assay to study chromosomal reconfiguration of the human GH1 locus by energy homeostasis
|Vakili H,Jin Y,Cattini PA||The Journal of clinical investigation (124:5002)||2014|
Developmentally regulated GTP-binding protein 2 ameliorates EAE by suppressing the development of TH17 cells.
PA1-844A was used in ChIP assay to study the role of suppressed Th17 cell development in the mechanism by which transgenic DRG2 overexpression protects against experimental autoimmune encephalomyelitis
|Ko MS,Kim HJ,Kim HK,Yoon NA,Lee UH,Lee SC,Chung DK,Lee BJ,Suh JH,Cho WJ,Park JW||Clinical immunology (Orlando, Fla.) (150:225)||2014|
Vitamin D receptor binds to the ¿ germline gene promoter and exhibits transrepressive activity.
PA1-844A was used in ChIP assay to investigate the mechanism of the inhibition of IgE production mediated by calcitriol
|Milovanovic M,Heine G,Hallatschek W,Opitz B,Radbruch A,Worm M||The Journal of allergy and clinical immunology (126:1016)||2010|
Proteasomal degradation of retinoid X receptor alpha reprograms transcriptional activity of PPARgamma in obese mice and humans.
PA1-844A was used in ChIP assay to investigate the RXR alpha gene in obese mice and humans and its effect on transcriptional activity of PPAR gamma
|Lefebvre B,Benomar Y,Guédin A,Langlois A,Hennuyer N,Dumont J,Bouchaert E,Dacquet C,Pénicaud L,Casteilla L,Pattou F,Ktorza A,Staels B,Lefebvre P||The Journal of clinical investigation (120:1454)||2010|
Thyroid hormone-mediated negative transcriptional regulation of Necdin expression.
PA1-844A was used in ChIP assay to investigate the effect of thyroid hormone on Necdin expression
|Nygård M,Becker N,Demeneix B,Pettersson K,Bondesson M||Journal of molecular endocrinology (36:517)||2006|
A SUMOylation-dependent pathway mediates transrepression of inflammatory response genes by PPAR-gamma.
PA1-844A was used in chromatin immunoprecipitation to study the regulatory mechanism of PPAR gamma in adipogenesis and glucose homeostasis.
|Pascual G,Fong AL,Ogawa S,Gamliel A,Li AC,Perissi V,Rose DW,Willson TM,Rosenfeld MG,Glass CK||Nature (437:759)||2005|
PPARgamma regulates adipocyte cholesterol metabolism via oxidized LDL receptor 1.
PA1-844A was used in chromatin immunoprecipitation to investigate the role of the PPARgamma during the adipocyte cholesterol metabolism.
|Chui PC,Guan HP,Lehrke M,Lazar MA||The Journal of clinical investigation (115:2244)||2005|
Acute hypoxia affects P-TEFb through HDAC3 and HEXIM1-dependent mechanism to promote gene-specific transcriptional repression.
PA1-844A was used in immunocytochemistry to study the inhibitory effect of hypoxia on gene transcription and its mechanism
|Safronova OS,Nakahama K,Morita I||Nucleic acids research (42:8954)||2014|
mTORC1 controls fasting-induced ketogenesis and its modulation by ageing.
PA1-844A was used in immunohistochemistry to study the role of mTORC1 in ketogenesis and its age-dependent effect
|Sengupta S,Peterson TR,Laplante M,Oh S,Sabatini DM||Nature (468:1100)||2010|