OriGene

NDN Monoclonal Antibody (OTI5D1), TrueMAB™

View all (14) Necdin antibodies

Datasheet

Protocols

Questions & Answers

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Datasheet

Protocols

Questions & Answers

Tech Support

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NDN Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of HeLa cells using anti-NDN mouse monoclonal antibody (TA506975).

NDN Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human endometrium tissue within the normal limits using anti-NDN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506975)

NDN Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDN (RC200448, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-NDN. Positive lysates LY400887 (100 µg) and LC400887 (20 µg) can be purchased separately from OriGene.

Product Details

CF506975

Applications	Tested Dilution	Publications
Western Blot (WB)	1:4,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:150	-
Immunocytochemistry (ICC/IF)	1:100	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Clone	OTI5D1
Immunogen	Full length human recombinant protein of human NDN produced in HEK293T cell. View immunogen
Conjugate	Unconjugated
Form	lyophilized
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.3, with 8% trehalose
Contains	no preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

This intronless gene is located in the Prader-Willi syndrome deletion region. It is an imprinted gene and is expressed exclusively from the paternal allele.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Necdin; necdin homolog; necdin, melanoma antigen (MAGE) family member; necdin-like protein; Prader-Willi syndrome chromosome region

Gene Aliases: HsT16328; NDN; PWCR

UniProt ID: (Human) Q99608

Entrez Gene ID: (Human) 4692

Molecular Function: scaffold/adaptor protein

Function(s)

neuron migration respiratory system process transcription from RNA polymerase II promoter nervous system development axonal fasciculation central nervous system development negative regulation of cell proliferation glial cell migration post-embryonic development sensory perception of pain regulation of growth positive regulation of transcription from RNA polymerase II promoter neurotrophin TRK receptor signaling pathway axon extension multicellular organismal homeostasis genetic imprinting

Process(es)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding gamma-tubulin binding

NDN Monoclonal Antibody (OTI5D1), TrueMAB™

Product Details

CF506975

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

References (0)

Bioinformatics

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