Western blot of NDR1/STK38 in human spleen lysate was performed using 35ug protein in RIPA buffer. Lysates were probed with a NDR1/STK38 polyclonal antibody (Product# PA5-37923) at a dilution of 0.5ug/ml and incubated for 1 hour, followed by chemiluminescent detection.
|Tested species reactivity||Human, Rat|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence C-KPTVATSNHPET, from the C-terminus of STK38|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with dog, pig and cow based on sequence homology.
Stk38,also known as NUCLEAR DBF2-RELATED PROTEIN, contains 1 protein kinase domain that interacts with mob1 and mob2.The homodimeric s100b binds to two molecules of stk38. It is ubiquitously expressed with highest levels observed in peripheral blood leukocytes. Stk38 activated by binding of s100b which releases autoinhibitory n-lobe interactions, enabling atp to bind and the autophosphorylation of ser-281. Thr-444 then undergoes calcium- dependent phosphorylation by an upstream kinase. Interactions between phosphorylated Thr-444 and the n-lobe promote additional structural changes that complete the activation of the kinase. Autoinhibition is also released by the binding of mob1/mobkl1a and mob2/hcca2 to the n-terminal of stk38.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.