|Tested species reactivity||Bovine, Human, Mouse, Rat|
|Published species reactivity||Rat, Mouse, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Bovine Heart Complex I|
|Storage buffer||HEPES buffered saline|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-2 µg/10 ^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Western Blot (WB)||1.0 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Purification is near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
459100 detects a band at approximately 36 kDa.
Use human heart mitochondria as a positive control.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Twinkle overexpression prevents cardiac rupture after myocardial infarction by alleviating impaired mitochondrial biogenesis.
459100 was used in western blot to study alleviation of impaired mitochondrial biogenesis by twinkle overexpression preventing cardiac rupture after myocardial infarction
|Inoue T,Ikeda M,Ide T,Fujino T,Matsuo Y,Arai S,Saku K,Sunagawa K||American journal of physiology. Heart and circulatory physiology (311:H509)||2016|
Loss of CLPP alleviates mitochondrial cardiomyopathy without affecting the mammalian UPRmt.
459100 was used in western blot to study the alleviation of mitochondrial cardiomyopathy without affecting the mammalian UPRmt due to loss of CLPP
|Seiferling D,Szczepanowska K,Becker C,Senft K,Hermans S,Maiti P,König T,Kukat A,Trifunovic A||EMBO reports (17:953)||2016|
|Not Applicable||Not Cited||
Basal metabolic state governs AIF-dependent growth support in pancreatic cancer cells.
459100 was used in western blot to analyze control of AIF-dependent growth support in pancreatic cancer cells by basal metabolic state
|Scott AJ,Wilkinson AS,Wilkinson JC||BMC cancer (16:null)||2016|
|Not Applicable||Not Cited||
Loss of OMA1 delays neurodegeneration by preventing stress-induced OPA1 processing in mitochondria.
459100 was used in western blot to analyze delay of neurodegeneration by preventing stress-induced OPA1 processing in mitochondria by loss of OMA1
|Korwitz A,Merkwirth C,Richter-Dennerlein R,Tröder SE,Sprenger HG,Quirós PM,López-Otín C,Rugarli EI,Langer T||The Journal of cell biology (212:157)||2016|
Imbalanced OPA1 processing and mitochondrial fragmentation cause heart failure in mice.
459100 was used in western blot to study how OMA1 links mitochondrial morphology and cardiac metabolism.
|Wai T,García-Prieto J,Baker MJ,Merkwirth C,Benit P,Rustin P,Rupérez FJ,Barbas C,Ibañez B,Langer T||Science (New York, N.Y.) (350:null)||2015|
Cysteine dietary supplementation reverses the decrease in mitochondrial ROS production at complex I induced by methionine restriction.
459100 was used in western blot to investigate cysteine and methionine dietary supplementation in aging rodents.
|Gomez A,Gomez J,Lopez Torres M,Naudi A,Mota-Martorell N,Pamplona R,Barja G||Journal of bioenergetics and biomembranes (47:199)||2015|
CNC-bZIP protein Nrf1-dependent regulation of glucose-stimulated insulin secretion.
459100 was used in western blot to assess effects nuclear factor-erythroid 2-related factor 1 deficiency in beta-cells on beta-cell function and glucose homeostasis
|Zheng H,Fu J,Xue P,Zhao R,Dong J,Liu D,Yamamoto M,Tong Q,Teng W,Qu W,Zhang Q,Andersen ME,Pi J||Antioxidants and redox signaling (22:819)||2015|
Overexpression of TFAM or twinkle increases mtDNA copy number and facilitates cardioprotection associated with limited mitochondrial oxidative stress.
459100 was used in western blot to assess the role of mtDNA copy number in heart failure
|Ikeda M,Ide T,Fujino T,Arai S,Saku K,Kakino T,Tyynismaa H,Yamasaki T,Yamada K,Kang D,Suomalainen A,Sunagawa K||PloS one (10:null)||2015|
Cytosolic p53 inhibits Parkin-mediated mitophagy and promotes mitochondrial dysfunction in the mouse heart.
459100 was used in western blot to test if mitochondrial quality control mechanisms contribute to the development of cardiac dysfunction.
|Hoshino A,Mita Y,Okawa Y,Ariyoshi M,Iwai-Kanai E,Ueyama T,Ikeda K,Ogata T,Matoba S||Nature communications (4:null)||2013|
ß-Adrenergic stimulation does not activate p38 MAP kinase or induce PGC-1¿ in skeletal muscle.
459100 was used in western blot to test if the increases in PGC-α and mitochondrial biogenesis induced by endurance exercise are mediated by catecholamines.
|Kim SH,Asaka M,Higashida K,Takahashi Y,Holloszy JO,Han DH||American journal of physiology. Endocrinology and metabolism (304:E844)||2013|
The enzymatic activity of apoptosis-inducing factor supports energy metabolism benefiting the growth and invasiveness of advanced prostate cancer cells.
459100 was used in western blot to examine the contribution of apoptosis-inducing factor to prostate cancer.
|Lewis EM,Wilkinson AS,Jackson JS,Mehra R,Varambally S,Chinnaiyan AM,Wilkinson JC||The Journal of biological chemistry (287:43862)||2012|
Essential role of mitochondrial Ca2+ uniporter in the generation of mitochondrial pH gradient and metabolism-secretion coupling in insulin-releasing cells.
459100 was used in western blot to examine the contribution of the mitochondrial Ca(2+) uniporter to the regulation of oxidative phosphorylation and metabolism-secretion coupling in β-cells and rat pancreatic islets.
|Quan X,Nguyen TT,Choi SK,Xu S,Das R,Cha SK,Kim N,Han J,Wiederkehr A,Wollheim CB,Park KS||The Journal of biological chemistry (290:4086)||2015|
P150glued-associated disorders are caused by activation of intrinsic apoptotic pathway.
459100 was used in western blot to study the role of p150glued in apoptosis.
|Ishikawa K,Saiki S,Furuya N,Yamada D,Imamichi Y,Li Y,Kawajiri S,Sasaki H,Koike M,Tsuboi Y,Hattori N||PloS one (9:null)||2014|
Unexpected vascular enrichment of SCO1 over SCO2 in mammalian tissues: implications for human mitochondrial disease.
459100 was used in western blot to examine the tissue distribution of SCO1 and SCO2
|Brosel S,Yang H,Tanji K,Bonilla E,Schon EA||The American journal of pathology (177:2541)||2010|
Mitochondrial DNA toxicity compromises mitochondrial dynamics and induces hippocampal antioxidant defenses.
459100 was used in immunohistochemistry - paraffin section to investigate the effects of mitochondrial DNA damage on hippocampal neurons.
|Lauritzen KH,Cheng C,Wiksen H,Bergersen LH,Klungland A||DNA repair (10:639)||2011|