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NDUFB3 Antibody (PA5-103946) in ICC/IF
Immunocytochemistry analysis of NDUFB3 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with NDUFB3 polyclonal antibody (Product # PA5-103946) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-103946
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NDUFB3(Accession O43676), corresponding to amino acid residues M1-A38.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2853278
Product Specific Information
Antibody detects endogenous levels of total NDUFB3.
Target Information
The multisubunit NADH:ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of mitochondria.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CI-B12; Complex I-B12; NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 3, 12kDa; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3; NADH-ubiquinone oxidoreductase B12 subunit
Gene Aliases: 2700033I16Rik; AI415450; B12; CI-B12; NDUFB3
UniProt ID: (Human) O43676, (Mouse) Q9CQZ6
Entrez Gene ID: (Human) 4709, (Mouse) 66495, (Rat) 301427
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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