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Immunofluorescent analysis of NEF3 in cultures of adult neural cells using a NEF3 monoclonal antibody (Product # MA1-33723). The surrounding stellate red cells are stained with rabbit polyclonal Rat facial nucleus before RPCA-a-Int. These are apparently mitotic neuronal progenitor cells and express many other neuronal markers.
|Tested species reactivity||Human , Mammals , Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant C-terminal fragment (Rat).|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||1:500|
|Western Blot (WB)||1:2500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-33723 detects NEF3 from mammal, rat, human samples.
MA1-33723 has been successfully used in immunohistochemistry (frozen tissue), immunohistochemistry (paraffin tissue), Western blot applications.
The MA1-33723 immunogen is: Recombinant C-terminal fragment (Rat).
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Enhanced cisplatin cytotoxicity by disturbing the nucleotide excision repair pathway in ovarian cancer cell lines.
MA1-33723 was used in western blot to study the increased cytotoxicity of cisplatin following antisense knockdown of the nucleotide excision repair pathway in ovarian cancer cell lines
|Selvakumaran M,Pisarcik DA,Bao R,Yeung AT,Hamilton TC||Cancer research (63:1311)||2003|
Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells.
MA1-33723 was used in immunocytochemistry to transform human neuronal cells by means of human adenovirus and compare these cell lines with HEK 293 cells
|Shaw G,Morse S,Ararat M,Graham FL||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (16:869)||2002|
160 kDa neurofilament protein; major neuronal intermediate filament subunit; NEF3; neurofilament 3; neurofilament 3 (150kDa medium); neurofilament 3, medium; neurofilament M subunit; neurofilament medium polypeptide; neurofilament protein M; Neurofilament protein, middle polypeptide; neurofilament triplet M protein; neurofilament, medium polypeptide; neurofilament, medium polypeptide 150kDa; neurofilament-3 (150 kD medium); neurofilament-M; NF-M; NF-M c-terminus; NF-M protein; NFM
BOS_9093; NEF3; NEFM; NF-M; NF160; NF165; NFM