Immunofluorescent analysis of the neurofilament heavy, medium, and light chains in paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). Tissue sections were deparaffinized with xylene, and rehydrated with ethanol. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0) and microwaved for 8-15 min. Following antigen retrieval, tissues were washed with water and PBS, and then blocked in 0.3% BSA for 30 min at room temperature. Tissues were then probed with a neurofilament heavy, medium, and light chain monoclonal antibody (Product # 13-1300) in 0.3% BSA at a dilution of 1:20 for 1 hour at 37°C. Tissues were then incubated with a Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight 488 conjugate for 1 hour at 37°C (green). Nuclei (blue) were stained with DAPI. Images were taken at 40X magnification.
|Tested species reactivity||Bovine, Human, Mollusc, Mouse, Rabbit, Rat|
|Published species reactivity||Rat, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Adult Rat neurofilaments|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1-0.5 ug/ml|
|Western Blot (WB)||0.5-2 ug/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220kDa, with some variation in different species. NF-H polyclonal antibody can be used to identify neurons and their processes in tissue sections and in tissue culture and the antibody can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.
Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 180-220 kDa. NF-M is the medium molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 160 kDa. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Sustained release of neurotrophin-3 via calcium phosphate-coated sutures promotes axonal regeneration after spinal cord injury.
13-1300 was used in immunohistochemistry - paraffin section to learn promotion of axonal regeneration after spinal cord injury due to sustained release of neurotrophin-3 via calcium phosphate-coated sutures
|Hanna A,Thompson DL,Hellenbrand DJ,Lee JS,Madura CJ,Wesley MG,Dillon NJ,Sharma T,Enright CJ,Murphy WL||Journal of neuroscience research (94:645)||2016|
Cerebral and spinal cord tanycytic ependymomas in a young adult with a mutation in the NF2 gene.
13-1300 was used in immunohistochemistry - paraffin section to determine the immunohistochemical, ultrastructural and genetic features of a patient with tanycytic ependymoma and that has a heterozygous truncating mutation in the NF2 gene
|Kuga Y,Ohnishi H,Kodama Y,Takakura S,Hayashi M,Yagi R,Fukutome K,Matsushima K,Okamoto K,Taomoto K,Takahashi H||Neuropathology : official journal of the Japanese Society of Neuropathology (34:406)||2014|
Glutamate receptor expression and chronic glutamate toxicity in rat motor cortex.
13-1300 was used in immunohistochemistry to study the glutamate receptors on corticospinal neurons in acutely isolated rat motor cortex slices
|Young KC,McGehee DS,Brorson JR||Neurobiology of disease (26:78)||2007|
|Not Applicable||Not Cited||
Double immunolabeling of central nervous system atypical teratoid/rhabdoid tumors.
13-1300 was used in immunohistochemistry to characterize central nervous system atypical teratoid/rhabdoid tumors
|Bouffard JP,Sandberg GD,Golden JA,Rorke LB||Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc (17:679)||2004|
|Mouse||Not Cited||Conversion of myoblasts to physiologically active neuronal phenotype.||Watanabe Y,Kameoka S,Gopalakrishnan V,Aldape KD,Pan ZZ,Lang FF,Majumder S||Genes and development (18:889)||2004|
|Rat||Not Cited||Role of the sensory neuron cytoskeleton in second messenger signaling for inflammatory pain.||Dina OA,McCarter GC,de Coupade C,Levine JD||Neuron (39:613)||2003|
|Mouse||Not Cited||Mouse embryos cloned from brain tumors.||Li L,Connelly MC,Wetmore C,Curran T,Morgan JI||Cancer research (63:2733)||2003|