|ELISA (ELISA)||0.1-0.5 µg/mL|
|Immunoprecipitation (IP)||2-5 µg|
|Western Blot (WB)||0.5-2 µg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||See 2 publications below|
|Immunohistochemistry (IHC)||See 2 publications below|
|Western Blot (WB)||See 1 publications below|
|Immunofluorescence (IF)||See 2 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Species reactivity||Bovine, Hamster, Human, Mollusc, Mouse, Rabbit, Rat|
|Published species||Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Adult Rat neurofilaments|
|Contains||0.1% sodium azide|
This antibody reacts with the 160 and 200 kDa subunit proteins of human neurofilament. It specifically recognizes a nonphosphorylated epitope in the tail domain of NF-M+H. Dephosphorylation of NF-M+H will increase immunoreactivity with this antibody.
This antibody is suitable for immunohistochemical staining of Bouin's and alcohol-fixed paraffin-embedded or frozen tissue sections. To stain, incubate 30-60 minutes at room temperature or overnight at 4°C.
Neurofilaments are the intermediate (10-12 nm) filament proteins (IFPs) located specifically in neurons, and are composed predominantly of three classes/subunits of neurofilaments: NF-L (low molecular weight; 68 kDa), NF-M (medium molecular weight; 160 kDa), and NF-H (high molecular weight; 200 kDa), with some variation in different species. The neurofilaments are long helical proteins involved in the maintenance of neuronal caliber, which polymerize to form a rigid cytoskeleton in the neuron. Like most other IFPs, the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. This polymerized network is composed of all three filaments, and the stoichiometry of association varies during development. Neurofilaments are posttranslationally modified both by phosphorylation and glycosylation. Like other intermediate filament proteins, phosphorylation likely mediates neurofilament dynamics. However, how O-linked N-acetylglucosamine residues function to modify neurofilament function remains unclear. The NF-H polyclonal antibody can be used to identify neurons and their processes in tissue sections and in tissue culture and the antibody can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 160 kDa neurofilament protein; 200 kDa neurofilament protein; 68 kDa neurofilament protein; light molecular weight neurofilament protein; major neuronal intermediate filament subunit; Micro glutamic acid-rich protein; NEF3; NEFM; neurofilament 200kDa; Neurofilament 3; neurofilament 3, medium; Neurofilament heavy polypeptide; Neurofilament light polypeptide; neurofilament M subunit; Neurofilament medium polypeptide; neurofilament medium polypeptide 150kDa; neurofilament protein L; neurofilament protein M; neurofilament protein, light chain; Neurofilament protein, middle polypeptide; neurofilament subunit NF-L; Neurofilament triplet H protein; Neurofilament triplet L protein; Neurofilament triplet M protein; neurofilament, heavy polypeptide 200kDa; neurofilament, heavy polypeptide 200kDa-like; neurofilament, light polypeptide 68kDa; neurofilament, medium polypeptide; neurofilament, medium polypeptide 150kDa; neurofilament-3 (150 kD medium); neurofilament-3, NFH, NF-H, NHC, 200 kDa neurofilament protein, KIAA0845, Neurofilament heavy polypeptide, Neurofilament triplet H protein, NFL, NF-L, NLC, 68 kDa neurofilament protein, 70 kDa neurofilament protein, CMT1F, CMT2E, Neurofilament light polypeptide, neurofilament medium, neurofilament light, neurofilament heavy; neurofilament-M; NF-H; NF-L; NF-M; NFM; protein phosphatase 1, regulatory subunit 110
Gene Aliases: AI847934; BOS_9093; BOS_9100; CMT1F; CMT2CC; CMT2E; KIAA0845; mKIAA0845; NEF3; NEFH; NEFL; NEFM; NF-H; NF-L; NF-M; NF160; NF165; NF200; NF68; NFH; NFL; NFM; PPP1R110
Entrez Gene ID: (Rabbit) 100357177, (Human) 4744, (Bovine) 281348, (Human) 4747, (Bovine) 281347, (Rabbit) 100009343, (Human) 4741, (Rat) 24587, (Mouse) 380684, (Mouse) 18039, (Rat) 83613, (Mouse) 18040, (Rat) 24588
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