|ELISA (ELISA)||0.1-0.5 ug/ml|
|Western Blot (WB)||0.5-2 ug/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||See 2 publications below|
|Immunohistochemistry (IHC)||See 2 publications below|
|Western Blot (WB)||See 1 publications below|
|Immunofluorescence (IF)||See 1 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Tested Species reactivity||Bovine, Human, Mollusc, Mouse, Rabbit, Rat|
|Published species reactivity||Rat , Human , Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Adult Rat neurofilaments|
|Contains||0.1% sodium azide|
Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220kDa, with some variation in different species. NF-H polyclonal antibody can be used to identify neurons and their processes in tissue sections and in tissue culture and the antibody can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.
Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 180-220 kDa. NF-M is the medium molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 160 kDa. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 160 kDa neurofilament protein; 200 kDa neurofilament protein; 68 kDa neurofilament protein; light molecular weight neurofilament protein; major neuronal intermediate filament subunit; Micro glutamic acid-rich protein; NEF3; NEFM; neurofilament 200kDa; Neurofilament 3; neurofilament 3, medium; Neurofilament heavy polypeptide; Neurofilament light polypeptide; neurofilament M subunit; Neurofilament medium polypeptide; neurofilament medium polypeptide 150kDa; neurofilament protein L; neurofilament protein M; neurofilament protein, light chain; Neurofilament protein, middle polypeptide; neurofilament subunit NF-L; Neurofilament triplet H protein; Neurofilament triplet L protein; Neurofilament triplet M protein; neurofilament, heavy polypeptide 200kDa; neurofilament, light polypeptide 68kDa; neurofilament, medium polypeptide; neurofilament, medium polypeptide 150kDa; neurofilament-3 (150 kD medium); neurofilament-3, NFH, NF-H, NHC, 200 kDa neurofilament protein, KIAA0845, Neurofilament heavy polypeptide, Neurofilament triplet H protein, NFL, NF-L, NLC, 68 kDa neurofilament protein, 70 kDa neurofilament protein, CMT1F, CMT2E, Neurofilament light polypeptide, neurofilament medium, neurofilament light, neurofilament heavy; neurofilament-M; NF-H; NF-L; NF-M; NFM; protein phosphatase 1, regulatory subunit 110
Gene Aliases: AI847934; BOS_9093; BOS_9100; CMT1F; CMT2CC; CMT2E; KIAA0845; mKIAA0845; NEF3; NEFH; NEFL; NEFM; NF-H; NF-L; NF-M; NF160; NF165; NF200; NF68; NFH; NFL; NFM; PPP1R110
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