Immunoflourescent analysis of mixed neuron/glia cultures from newborn rat brain using NEFL polyclonal antibody (Product # PA1-32240). Samples were stained using an antibody to peripherin (green) and rabbit polyclonal antibody to NF-L RPCA-NF-L (red channel). A class of large neurons, like the one in the middle of this image, contain peripherin, while the majority of neurons and their processes contain NF-L and not peripherin. Interestingly, the periperin positive cells often contain a cytoplasmic inclusion next to the nucleus which stains for both peripherin and NF-L, and so appears golden in this kind of image. The blue channel reveals the localization of DNA.
|Tested species reactivity||Bovine, Human, Mammal, Mouse, Pig, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Native NF-L purified from pig spinal cord|
|Storage buffer||whole serum|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:1000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:1000|
|Western Blot (WB)||1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-32240 detects NEFL from rat, human, mouse, porcine samples.
PA1-32240 has been successfully used in immunohistochemistry (frozen tissue), immunohistochemistry (paraffin tissue), Western blot applications.
The PA1-32240 immunogen is: Native NF-L purified from pig spinal cord
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.