|ELISA (ELISA)||0.1-0.5 ug/ml|
|Immunohistochemistry (IHC)||1:20 - 1:100|
|Immunoprecipitation (IP)||2-5 ug|
|Western Blot (WB)||0.5-2 ug/mL|
|Immunocytochemistry (ICC)||See 1 publications below|
|Immunohistochemistry (IHC)||See 3 publications below|
|Miscellaneous PubMed (MISC)||See 3 publications below|
|Western Blot (WB)||See 5 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 1 publications below|
|Immunofluorescence (IF)||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat , Mouse , Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Specifically recognizes a phosphate-independent epitope on NF-L.|
|Contains||0.1% sodium azide|
13-0400 detects the neurofilament, light chain in human, rat, and mouse samples.
Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa.
Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220kDa, with some variation in different species. NF-H polyclonal antibody can be used to identify neurons and their processes in tissue sections and in tissue culture and the antibody can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 68 kDa neurofilament protein; light molecular weight neurofilament protein; medium polypeptide 150kDa; NEF3; neurofilament; neurofilament 3; Neurofilament light polypeptide; neurofilament medium polypeptide; neurofilament protein L; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L protein; neurofilament triplet M protein; neurofilament, light polypeptide 68kDa; neurofilament-3 (150 kD medium); NF-L; NFM; protein phosphatase 1, regulatory subunit 110
Gene Aliases: AI847934; CMT1F; CMT2E; NEFL; NF-L; NF68; NFL; PPP1R110
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